การศึกษารูปแบบลำดับนิวคลีโอไทด์บริเวณยีน 16S rRNA ของแบคทีเรีย Paenibacillus polymyxa โดยใช้ความแตกต่างของนิวคลีโอไทด์หนึ่งตำแหน่ง
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Abstract
Abstract
Paenibacillus polymyxa plays a major role in the production of xylanase enzyme, which can be potentially applied in paper and animal feed industries. It is necessary to study the identification of the bacteria for preserving bacterial cultures and effectively bacterial utilization. In this study, we analyzed nucleotide sequences and determined the single nucleotide polymorphisms (SNPs) in 16S rRNA gene of P. polymyxa. The results showed that 10 strains of P. polymyxa contained 11 to 14 rRNA gene copies of 16S rRNA gene, which are located within each genome. The polymorphic sites were found among the multiple 16S rRNA genes and the alignment results showed that 29 variable sites were detected. The region ranging from 465 to 488, which can be amplified using universal 16S rRNA gene primers. This region could be divided into 2 nucleotide sequence patterns (pattern 1 and pattern 2). The pattern 1 could be used to identify Paenibacillus sp. BTK01 as P. polymyxa BTK01 that corresponded with the distance correlation coefficient (0.000 to 0.0017) of 10 reference strains. Thus, the patterns represented the variation within species, which could be used for discrimination of P. polymyxa at subspecies level. In conclusion, the results suggest that these 2 sequence patterns of 16S rRNA gene may improve the 16S rRNA database for species and subspecies identification of P. polymyxa.
Keywords: Paenibacillus; SNP; 16S rRNA gene; identification; subspecies
Article Details
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
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