In vitro Mineral Nutrients and Sucrose Affecting Growth and Development of Micropropagated Red Raspberry Shoots
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Abstract
Red raspberry (Rubus idaeus Linn.) has been grown for fruit production in the north of Thailand by the Royal Project foundation. Mass production of red raspberry plant stock is needed for commercial fruit production. This alternative crop might be interesting for increased incomes of upland farmers. The aim of this study was to investigate the effects of mineral nutrients and sucrose on plant growth and development of micropropagated red raspberry ‘Amity’. Previous studies showed that minerals in Murashige and Skoog (MS) medium were not suitable for this plant. Therefore, in the current study, the effects of the mineral components in three groups; nitrogen, mesos and micronutrients were tested as the relative concentrations compared to MS (0.5x, 1.0x, 1.5x and 2.0x MS) and then sucrose concentrations were optimized. Plants were evaluated for shoot quality, leaf color, multiplication and shoot length. The results showed that reduced mesos negatively affected plant quality, leaf color and shoot number. The modified MS suitable for this cultivar was half the MS nitrogen concentration and twice the MS mesos concentration. The leaf color measured by SPAD meter for greenness correlated with the chlorophyll content of the leaf tissues. The best sucrose concentration for improving growth of micropropagated red raspberry shoots was 1.5 %.
Article Details
บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
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