Biofilm Formation, Proteinase and Phospholipase Activities of Candida parapsilosis Isolated from Environment

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Srisuda Pannanusorn
Natnapat Nakkao
Nattida Cholnakasem


Candida parapsilosis, although, is a human commensal of epithelial and mucosal tissues. This species is also frequently isolated from environments. C. parapsilosis is notable for the ability to form biofilms on medical devices. Therefore, it is frequently associated with nosocomial outbreaks. In addition, ability of C. parapsilosis producing secrete proteinase and phospholipase can causes more severe infection. The present study aimed to determine biofilm formation using crystal violet staining and hydrolytic enzyme production using spot assay on relevant agar medium of C. parapsilosis isolated from environment. In total, 8 of 30 yeast isolates (26.66 %) obtained from environment were C. parapsilosis. Biofilm formation of the isolated C. parapsilosis was variable. All of the isolates revealed the higher biofilm formation at room temperature (30 °C) compared to 37 °C. All the isolates showed moderate to high activity of proteinase but no activity of phospholipase. In contrast to biofilm formation, proteinase production of the isolates was increased at 37 °C. In conclusion, temperature is one important factor affecting biofilm formation and proteinase production. The formation of the biofilm is possibly beneficial for the isolates to live in the hostile environment. However, production of proteinase may play important role for C. parapsilosis to cause infection in human.


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Pannanusorn, S., Nakkao, N., & Cholnakasem, N. (2019). Biofilm Formation, Proteinase and Phospholipase Activities of Candida parapsilosis Isolated from Environment. Thai Journal of Science and Technology, 9(1), 129–141.
Author Biographies

Srisuda Pannanusorn

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120

Natnapat Nakkao

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120

Nattida Cholnakasem

Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, Pathum Thani 12120


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