Morphological and molecular identification of the predatory nematode, Mononchoides (Nematoda: Diplogasterida) from Thailand

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Arunee Suwanngam
Anongnuch Sasnarukkit
Raktapa Phonginsee
Buncha Chinnasri
Srimek Chowpongpang

Abstract

Predatory nematodes; Mononchoides are the beneficial nematodes that feed on small organisms or other nematodes. Mononchoides population collected from guava orchards and para rubber plantation in Thailand was characterized by morphological and molecular techniques. Mononchoides specie is characterized by a dorsal claw-like tooth; 7-8 µm wide and 3-4 µm long, long body (540-650 µm), didelphic-amphidelphic reproductive system and a relatively short filiform tail (40-50µm long). Genetic analysis of Mononchoides based on species-specific primers and sequence on the ribosomal DNA. This molecular diagnosis using Mnc1, Mnc3 and Mn28s primers were confirmed by nucleotide sequences that shared 100% similarity with the known nematode sequences (FJ661080, LC210629, KT884893, LN827618, KJ877211, KF151166, KP067833) in GenBank database. The Mononchoides-specific primers have been developed to offered a rapid species identification approach for further analysis and management.  

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How to Cite
Suwanngam, A., Sasnarukkit, A., Phonginsee , R., Chinnasri, B., & Chowpongpang, S. (2021). Morphological and molecular identification of the predatory nematode, Mononchoides (Nematoda: Diplogasterida) from Thailand. Thai Journal of Science and Technology, 10(2). https://doi.org/10.14456/tjst.2021.14
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วิทยาศาสตร์ชีวภาพ

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