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The purpose of this study was screening the phosphate solubilizing bacteria (PSB) to enhance plant growth in agroecosystem. Forty-one rhizosphere bacterial strains were isolated from bamboo rhizosphere soil at Pathum Thani province with completely randomized design (CRD). Ten out of 41 strains showed phosphate solubilization capacity on Pikovskaya’s agar. The PSB28 and PSB15 were the most efficient bacterial isolates for phosphate solubilization as showed the largest clear zone diameter with 2.13±0.07 and 1±0.26 cm, respectively (p<0.05). Gram stain and hypersensitive response test revealed that PSB28 and PSB15 were Gram negative and Gram positive, respectively. Therefore, PSB15 was selected to test for ability to solubilized phosphate with different phosphate resource including calcium phosphate (Ca3(PO4)2), rock phosphate, and ammonium dihydrogen phosphate ((NH4)H2PO4). The result showed that calcium phosphate (Ca3(PO4)2) was most capable source of phosphate dissolved by PSB15 as showed available phosphorus in total amount of 69.67±1.8, 60.80±8.66, and 59.96±8.31 mg/kg at 7, 14, and 21 days, respectively. For identification of PSB15, the 16S rRNA of gene sequencing was applied with two universal primers, 27F (5’-AGA GTT TGA TCM TGG CTC AG-3’) and 1492R (5’-CGG TTA CCT TGT TAC GAC TT-3’). The results of analysis showed the yielded of sequences was similarity score of ≥ 98% with the sequences of Bacillus paramycoides in GenBank. When PSB15 was applied with rice seedling, it showed high efficiency on plant growth promotion. It can be mixed used or substituted chemical fertilizer to enhance available phosphorus in soil and increase plant growth. These results revealed that application of phosphate solubilizing bacteria, B. paramycoides PSB15 with phosphate resource in agroecosystem can be an alternative method to reduce the use of synthetic fertilizer.
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Barea J.M., M.J. Pozo, R. Azcón and C. Azcón-Aguilar. 2005. Microbial co-operation in the rhizosphere. J. Exp. Bot. 56: 1761-1778.
Bertin C., H.W. Yang and L.A. Weston. 2003. The role of root exudates and allelochemicals in the rhizosphere. Plant Soil 256: 67-83.
Bray II, R.H. and L.T. Kurtz. 1945. Determination of Total, Organic and Available Forms of Phosphorus in Soils. Soil Sci. 59: 39-45.
Dadarwal, K. R. 1997. Microorganisms for sustainable crop production. Jodhpur. 293-308.
Department of Land Development. 2009. Plant and Fertilizer Analysis Protocol Manual. Manual No. OSD-03. Department of Land Development. Bangkok. (in Thai)
Gerke, L. 1992. Phosphate, aluminum and iron in the soil solution of three different soils in relation to varying concentrations of citric acid. Z. Pflanzenernahr. Bodenk. 155: 17-22.
Gerretsen, F.C. 1948. The Influence of Microorganisms on the Phosphate Intake by the Plant. Plant and Soil 1: 51-81.
Jantarasombat, W., N. Pasada, M. Peerawatchara, and V. Rophandung.2009.Screening of Phosphate Solubilizing Microorganism for agricultural benefits.Office of Soil Resources Survey and Research. Department of Land Development. (in Thai)
Junrungreang, S., B. Rossopa, and K. Sajjaphan. 2010. Effect of phosphate-solubilizing bacteria, Burkholderia sp. strain Rs01, on growth of Insee 2 sweet corn. Master Thesis Department of Soil Science. Kasetsart University. (in thai)Alexander, M. 1969. Natural Selection of Microorganisms in Extreme Environments. Plant and Soil 43(2): 211-235. Final report. NASA-CR-105657. (in Thai)
Kanghae, P., S. Jaikawin, A. Hantanapong, S. Pinmanee, and W. Homhual. 2018. Diversity and efficiency of phosphate solubilizing bacteria from the rhizosphere of rice in the upper northern Thailand. Thai Rice Research Journal 9(1): 46-59. (in Thai)
Karnamnuey, N. 2007. Study of Diversity and Efficacy of Bacillus sp. For solubilizing Inorganic Phosphate. Master Thesis Department of Biotechnology. Kasetsart University. (in Thai)
Kositratana, W. 1994. Bacteria causing plant disease. Faculty of Agriculture Kasetsart University, Bangkok. 87-89. (in Thai)
Mala, T. 1991. The use of microorganisms that are soluble in phosphate rock to increase the usefulness of Phosphates and plant yields. Research report of Kasetsart University. (in Thai)
Mala, T. 2003. Organic Fertilizer and Bio Fertilizer: Production technique and Application. Department of Soil Science, Faculty of Agriculture Kasetsart University. Bangkok. 300p. (in Thai)
Mala, T. 2007. Organic Fertilizers and Biofertilizers: Production Techniques and Utilization. Faculty of Agriculture, Kasetsart University. Kasetsart University Press, Bangkok. (in Thai)
Salehrastin N., 1999. Biological Fertilizers. Soil and Water Research Institute of Iran. Sci. J. Soil and Water. 12: 3.
Schaad, N.W. 1988. Laboratory Guide for Identification of Plant Pathogenic Bacteria. Bacteriology Committee of American Phytopathological Society, St. Paul, Minnesota.
Somsa, K. 2006. Phosphorus. Agricultural Chemical Materials and Products Testing Group. Department of Science Service. (in Thai)
Srichai, N. 2003. Isolating and Screening of Phosphate Solubilizing Bacteria from soil. Master Thesis Department of Biotechnology. Chiang Mai University. (in Thai)
Suthon, W., Y. Khaosumain and C. Thanarut. 2016. Development of The Appropriate Technology on Plant Nutrition Management for Organic ‘Chokanan’ Mango Production. Mae-Jo University. (in Thai)
Tilak K.V.B.R., N. Ranganayaki, K.K. Pal, R. De, A.K. Saxena, C.S. Nautiyal, S. Mittal, A.K. Tripathi and B.N. Johri. 2005. Diversity of plant growth and soil health supporting bacteria. Curr. Sci. 89: 136-150.
Uren N.C. 2000. Types, amounts and possible functions of compounds released into the ehizosphere by soil grown plants, pp 19-40. In R. Pinton, Z. Varanini, P. Nannipieri, eds. The Rhizosphere: Biochemistry and Organic Substances at the Soil Interface. New York: Marcel, Dekker.
Wimat, N., P. Pinjai, and N. Jittamart. 2020. Phosphorus Availability in Soil and Sugarcane Growth by Phosphate Solubilizing Bacteria. King Mongkut’s Agr. J. 38(4): 477-488. (in Thai)
Woo, P.C.Y., S.K.P. Lau, J.L.L. Teng, H. Tse, and K.Y. Yuen. 2008. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin. Microbiol. Infect., 14(10): 908–934.
Yadav, K.S. and K.R. Dadarwal. 1997. Phosphate solubilisation and mobilization through soil microorganisms. In K.R. Dadarwal (Ed.), Biotechnological Approaches in Soil Microorganisms for Sustainable Crop Production, p. 351.
Yahya, A.I. and S.K. Al-Azwi. Occurance of Phosphate-solubilizing Bacteria in Some Iraqi Soils. Plant and Soil 117: 135-141.
Yiam-on, T., N. Riddech, P. Jaisil, and S. Boonlue. 2012. Growth promotion of sugarcane by phosphate solubilizing bacteriain green house condition. Khon Kaen Arg. J. 40(3): 185-193. (in Thai)