การวิเคราะห์ความไม่สมดุลของลิงเกจภายในยีน Plant U-box ในอ้อย
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Abstract
Understanding linkage disequilibrium (LD) is important for planning breeding strategies to target beneficial alleles for agronomic traits. In the present study, we analyzed the LD pattern of U-box domain-containing protein35 gene (SaPUB35) in 68 exotic sugarcane collections and 73 sugarcane breeding lines. PacBio single molecule, real-time (SMRT) sequencing was performed to identify 126 single nucleotide polymorphisms (SNPs) and 50 Insertion/deletions (InDels) in 5731 bp of SaPUB35. The exotic sugarcane population has more variants than the breeding line population. Pairwise LD analysis revealed that mean LD, at a threshold of squared allele frequency correlations (r2 ) = 0.1, extends to at least 1500 bp in the breeding line population but is eroded to 1400 bp in exotic sugarcane population. This implies a signature of a selective sweep for a beneficial mutation during the selection processes of sugarcane breeding. Uncovering allelic erosion and a diversity bottleneck due to modern sugarcane breeding will be useful for understanding sugarcane breeding targets and in devising future breeding strategies to target beneficial alleles currently not pursued.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
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