Genetic Relationship Assessment of Apples Using HAT-RAPD Technique
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Abstract
High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) technique was used for genetic relationship assessment of 14 apple samples that sold in Thailand. Thirty-one random primers out of seventy-two were successful for DNA amplification. Then 20 random primers which gave clear amplified products were selected for DNA fingerprinting. The result showed significant differences among 14 samples and also showed some bands in each sample. Moreover, this research found 3 random primers which tended to be DNA markers for apple identification. A dendrogram constructed based on polymorphic bands showed genetic similarities among apples and separated them into 3 clusters with similarity coefficients ranging from 0.57 to 0.86.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
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