Assessment of Genetic Diversity and Relationships of Anoectochilus burmannicus and Related Species in Thailand Using ISSR Marker
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Abstract
Anoectochilus burmannicus, a terrestrial orchid, has a potential as medicinal plant. Because its habitat is confined and deforested, it is diminished in nature. Therefore, its habitat located in Chiang Mai province, Thailand, is protected as a conservation area. However, genetic diversity of this plant in the area is still unknown. The objective of the study was to assess the genetic relationships and diversity of A. burmannicus and related species collected from northern Thailand. Thirty-three DNA samples of A. burmannicus, A. albolineatus, A. roxburghii, A. elwesii and O. lanceolatus were analyzed using 7 inter-simple sequence repeat (ISSR) primers. A total of 82 bands were obtained with 98.9% polymorphism. All 5 studied species were clearly separated and clustered into 2 groups. Nei’s genetic diversity (H) and Shannon information index (I) of A. burmannicus were 0.22 and 0.33, respectively, which revealed the moderate genetic diversity. For A. albolineatus, A. roxburghii and A. elwesii, H and I were ranged from 0.12-0.15 and 0.19-0.23, respectively. While, genetic diversity of O. lanceolatus was low (H=0.07, I=0.10). The results shown here were the first report about genetic relationships and diversity of Anoectochilus sp. in Thailand. These findings might provide essential information for developing genetic conservation strategy in these studied species in Thailand.
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บทความที่ได้รับการตีพิมพ์เป็นลิขสิทธิ์ของคณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ข้อความที่ปรากฏในแต่ละเรื่องของวารสารเล่มนี้เป็นเพียงความเห็นส่วนตัวของผู้เขียน ไม่มีความเกี่ยวข้องกับคณะวิทยาศาสตร์และเทคโนโลยี หรือคณาจารย์ท่านอื่นในมหาวิทยาลัยธรรมศาสตร์ ผู้เขียนต้องยืนยันว่าความรับผิดชอบต่อทุกข้อความที่นำเสนอไว้ในบทความของตน หากมีข้อผิดพลาดหรือความไม่ถูกต้องใด ๆ
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