ฤทธิ์ต้านจุลินทรีย์และต้านอนุมูลอิสระของสารสกัดจากราเอนโดไฟท์และพืชเจ้าบ้าน (บัวหลวง)
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ม.ค. 3, 2018
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ราเอนโดไฟท์ บัวหลวง ฤทธิ์ต้านจุลินทรีย์ ฤทธิ์ต้านอนุมูลอิสระ
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บทคัดย่อ
บทคัดย่อ
งานวิจัยนี้มีวัตถุประสงค์เพื่อประเมินฤทธิ์ต้านจุลินทรีย์และต้านอนุมูลอิสระของสารสกัดหยาบจากราเอนโด-ไฟท์และพืชเจ้าบ้าน (บัวหลวง) แยกราเอนโดไฟท์ได้ทั้งหมด 31 ไอโซเลท จากชิ้นตัวอย่าง 480 ชิ้น นำราเอนโดไฟท์ทั้งหมดไปเลี้ยงในอาหารเหลว PDB จากนั้นสกัดสารจากราเอนโดไฟท์และพืช (กลีบดอกและใบ) ด้วยเอทิลอะซิเตท และเมทานอล นำสารสกัดหยาบจากราเอนโดไฟท์จำนวน 93 สาร และสารสกัดจากบัวหลวงจำนวน 4 สาร มาทดสอบฤทธิ์ต้านจุลินทรีย์และต้านอนุมูลอิสระโดยวิธี colorimetric broth microdilution และ DPPH free radical scavenging ตามลำดับ พบว่าสารสกัดจากราเอนโดไฟท์มีประสิทธิภาพดีกว่าสารสกัดจากบัวหลวง โดยสารสกัดจากเส้นใยที่สกัดด้วยเอทิลอะซิเตท (CE) ของราเอนโดไฟท์ไอโซเลท FL020 (Curvularia geniculata) สามารถยับยั้ง MRSA และ S. aureus ได้ดีที่สุดให้ค่า MIC เท่ากับ 4 และ 8 ไมโครกรัมต่อมิลลิลิตร ตามลำดับ ในขณะที่สารสกัดเส้นใยที่สกัดด้วยเมทานอล (CM) จากราเอนโดไฟท์ ไอโซเลท FL014 (Curvularia sp.) มีฤทธิ์ต้านอนุมูลอิสระ DPPH ดีที่สุด ให้ค่า IC50 เท่ากับ 0.02 มิลลิกรัมต่อมิลลิลิตร จากการศึกษานี้ยืนยันว่าราเอนโดไฟท์จากบัวหลวงเป็นแหล่งของสารเมทาบอไลท์ทุติยภูมิที่มีศักยภาพ
คำสำคัญ : ราเอนโดไฟท์; บัวหลวง; ฤทธิ์ต้านจุลินทรีย์; ฤทธิ์ต้านอนุมูลอิสระ
Article Details
บท
Biological Sciences
References
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[25] Ming-Zhu, Z., Wu, W., Li-Li, J., Ping-Fang, Y. and Ming-Quan, G., 2015, Analysis of flavonoids in Lotus (Nelumbo nucifera) leaves and their antioxidant activity using macroporous resin chromatography coupled with LC-MS/MS and antioxidant biochemical assays, Molecules 20: 10553-10565.
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[28] Bhardwaj, A., Sharma, D., Jadon, N. and Agarwal, P.K., 2015, Antimicrobial and phytochemical screening of endophytic fungi isolated from spike of Pinus roxburghii, AC Microb. 6: 1-9.
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[32] Nagda, V., Gajbhiye, A. and Kumar, D., 2017, Isolation and characterization of endophytic fungi from Caltropi sprocera for their antioxidant activity, Asian J. Pharm. Clin. Res. 10: 254-258.
[33] Sunder, J., Dingh, D.R., Jeeyakumar, S., Kundu, A. and De, A.K., 2011, Antibacterial activity in solvent extract of different parts of Morinda citrifolia plant, J. Pharm. Sci Res. 3: 1404-1407.
[34] Abdelfadel, M.M., Khalaf, H.H., Sharoba, A.M. and Assous, M.T.M., 2016, Effect of extraction methods on antiocidant and antimicrobial activities of some spices and herbs extracts, J. Food Technol. Nutr. Sci. 1: 1-14.
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[37] Valle, D.L., Cabrera, E.C., Puzon, J.J.M. and Rivera, W.L., 2016, Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of Philippine Piper betle L. on clicical isolates of Gram-positive and Gram-negative bacteria with transferable multiple drug resistance, PLos ONE 11: e0146349.
[2] Newman, D.J., Cragg, G.M. and Snader, K.M., 2003, Natural products as sources of new drugs over the period 1981-2002, J. Nat. Prod. 66: 1022-1037.
[3] Gao, X., Zhou, H., Xu, D., Yu, C., Chen, Y. and Qu, L., 2005, High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivation-independent approach, FEMS Microbiol. Lett. 249: 255-266.
[4] Alva, P., McKenzie, E.H.C., Pointing, S.B., Pena-Muralla, R. and Hyde, K.D., 2002, Do sea grasses harbor endophytes: Fungi in marine environments, edited by K.D. Hyde, Fungal Diversity Res. Ser. 7: 167-178.
[5] Aly, A.H., Debbab, A., Kier, J. and Proksch, P., 2010, Fungal endophytes from higher plants: A prolific source of phytochemicals and other bioactive natural products, Fungal Divers. 41: 1-16.
[6] Huang, Y., Wang, J., Li, G., Zheng, Z. and Su, W., 2001, Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxusmairei, Cephalaxusfortunei and Torreyagrandis, FEMS Immunol. Med. Microbiol. 31: 163-167.
[7] Phongcharoen, W., Rukachaisirikul, V., Phongpaichit, S. and Sakayaroj, J., 2007, A new dihydrobenzofuran derivative from the endophytic fungus Botryosphaeria mamane PSU-M76, Chem. Pharm. Bull. 55: 1404-1405.
[8] Rukachisirikul, V., Sommart, U., Phongpai-chit, S., Sakayaroj, J. and Kirtikara, K., 2008, Metabolites from the endophytic fungus Phomopsis sp. PSU-D15. Phytochemistry 69: 783-787.
[9] Kanabkaew, T. and Puetpaiboon, U., 2004, Aquatic plants for domestic wastewater treatment: Lotus (Nelumbo nucifera) and Hydrilla (Hydrilla verticillata) systems, Songklanakarin J. Sci. Technol. 26: 749-756.
[10] Bhardwaj, A. and Modi, K.P., 2015, A review on therapeutic potential of Nelumbo nucifera (Gaertn): The sacres lotus, IJPSR. 7: 42-54.
[11] Supaphon, P., Phongpaichit, S., Rukachaisirikul, V. and Sakayaroj, J., 2013, Antimicrobial potential of endophytic fungi derived from three seagrasses (Cymodocea serrulata, Halophila ovalis and Thalassia hemprichii) from Thailand, PLoS ONE 8(8): e72520. (doi: 10.1371/ journal.pone.0072520).
[12] Premianu, N. and Jaynthy, C., 2014, Antioxidant activity of endophytic fungi isolated from Lannea coromendalica, Int. J. Res. Pham. Sci. 5: 304-308.
[13] O'Donnell, K. Cigelnik, E. Weber, N.S., Trappe, J.M., 1997, Phylogenetic relationships among Ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis, Mycologia 89: 48-65.
[14] Matin, K.J. and Rygiewicz, P.T., 2005, Fungal-specific PCR primers developed for analysis ITS region of environmental DNA extracts, BMC Microbiol. 5: 1-11.
[15] Hall, T., 2005, BioEdit: Biological Sequence Alignment Editor for Windows 95/98/NT/ XP, Available Source: http://www.mbio. ncsu.edu/bioedit/page1.html.
[16] Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994, Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and wwight matrix choice, Nucl. Acids Res. 22: 4673-4680.
[17] Swofford, D.L., 2002, PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4, Sinauer Associates, Sunderland, Massachusetts.
[18] Kishino, H. and Hasegawa, M., 1989, Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea, J. Mol. Evol. 29: 170-179.
[19] Stamatakis, A., 2014, RAxML version 8: A tool for phylogenetic analysis and post analysis of large phylogenies, Bioinfor-matics 30: 1312-1313.
[20] Nylander, J.A.A., 2004, MrModeltest v. 2.0, Evolutionary Biology Centre, Uppsala University, Sweden.
[21] Owen, N.L. and Hundley, N., 2004, Endophytes-the chemical synthesizers inside plants, Sci. Prog. 87: 79-99.
[22] Won, K.J., Lin, H.Y., Jung, S., Cho, S.M., Shin, H.C., Bae, Y.M., Lee, S.H., Kim, H.J., Joen, B.H. and Kim, B., 2012, Antifungal miconazole induces cardiotoxicity via inhibition of APE/REF-1-related pathway in rat neonatal cardiomyocytes, J. Toxicol. Sci. 126: 289-305.
[23] Wilson, W.L., 1998, Isolation of endophytes from seagrasses from Bermuda, M.Sc. Thesis, University of New Brunswick, Canada.
[24] Arjun, P., Priya, S.M., Sivan, P.S.S., Krishnamoorthy, M. and Balasubramanian, K., 2012, Antioxidant and antimicrobial activity of Nelum bonucifera Gaertn. leaf extracts, J. Acad. Indus. Res. 1: 15-18.
[25] Ming-Zhu, Z., Wu, W., Li-Li, J., Ping-Fang, Y. and Ming-Quan, G., 2015, Analysis of flavonoids in Lotus (Nelumbo nucifera) leaves and their antioxidant activity using macroporous resin chromatography coupled with LC-MS/MS and antioxidant biochemical assays, Molecules 20: 10553-10565.
[26] Shukla, K. and Chaturvedi, N., 2016, In vitro antioxidant properties of different parts of Nelumbo nucifera Gaertn. IJAPBC. 5: 196-201.
[27] Kharwar, R.N., Verma, S.K., Mishra, A., Gond, S.K., Sharma, V.K., Afreen, T. and Kumar, A., 2011, Assessment of diversity, distribution and antibacterial activity of endophytic fungi isolated from a medicinal plant, Adeocalymma alliaceum Miers, Symbiosis 55: 39-46.
[28] Bhardwaj, A., Sharma, D., Jadon, N. and Agarwal, P.K., 2015, Antimicrobial and phytochemical screening of endophytic fungi isolated from spike of Pinus roxburghii, AC Microb. 6: 1-9.
[29] Yadav, M., Yadav, A. and Yadav, J.P., 2014, In vitro antioxidant activity and total phenolic content of endophytc fungi isolated from Eugenia jambolana Lam, Asian Pac. J. Trop. Med. 7: 256-261.
[30] Nayak, B.K., 2014, Endophytic fungal enumeration from various leaf samples of a medicinal plant: Ziziphus mauritiana, Int. J. Pharm. Tech. Res. 7: 344-348.
[31] Chen, J.M., Ling, C. and Lu-Ping, Q., 2016, A friendly relationship between endophytic fungi and medicinal plants: A systematic review, Front Microbio. 7: (doi: 10.3389/ fmicb.2016.00906)
[32] Nagda, V., Gajbhiye, A. and Kumar, D., 2017, Isolation and characterization of endophytic fungi from Caltropi sprocera for their antioxidant activity, Asian J. Pharm. Clin. Res. 10: 254-258.
[33] Sunder, J., Dingh, D.R., Jeeyakumar, S., Kundu, A. and De, A.K., 2011, Antibacterial activity in solvent extract of different parts of Morinda citrifolia plant, J. Pharm. Sci Res. 3: 1404-1407.
[34] Abdelfadel, M.M., Khalaf, H.H., Sharoba, A.M. and Assous, M.T.M., 2016, Effect of extraction methods on antiocidant and antimicrobial activities of some spices and herbs extracts, J. Food Technol. Nutr. Sci. 1: 1-14.
[35] Beveridge, T.J., 1999, Structure of Gram-negative cell walls and their derived membrane vesicles, J. Bacteriol. 181: 4725-
4733.
[36] Modarresi-Chahardehi, A., Ibrahim, D., Fariza-Sulaiman, S. and Mousavi, L., 2012, Screening antimicrobial activity of various extracts of Urtica dioica, Rev. Biol. Trop. 60: 1567-1576.
[37] Valle, D.L., Cabrera, E.C., Puzon, J.J.M. and Rivera, W.L., 2016, Antimicrobial activities of methanol, ethanol and supercritical CO2 extracts of Philippine Piper betle L. on clicical isolates of Gram-positive and Gram-negative bacteria with transferable multiple drug resistance, PLos ONE 11: e0146349.