ประสิทธิภาพของราเอนโดไฟท์ในการยับยั้งเชื้อสาเหตุโรคไหม้ของข้าว
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Abstract
Abstract
This research aimed to investigate the potential of fungal endophytes from Nymphaea lotus and their crude extracts to control M. oryzae, the pathogen that cause rice blast disease and identified the most effective of these fungi by using molecular technique. A total of 40 fungal isolates were obtained from 300 segments of N. lotus and identified based on their morphological characteristics. Seventeen isolates were selected from their morphological appearance on potato dextrose agar and tested for their antagonistic potential against eight strains of M. oryzae by dual culture plate method. Four fungal isolates (LW_FS055, LW_L027, LP_LS026 and LW_L002) showed very strong inhibitory action (> 85 % inhibition) over the growth of M. oryzae 33 during dual culture assay. Then, these active isolates were cultured in potato dextrose broth for 3 weeks and extracted with ethyl acetate. The extracts of the most potential isolates were also evaluated for antifungal activity against plant pathogens by using agar dilution plate method at final concentration of 5, 1 and 0.2 mg/ml, respectively. At 5 and 1 mg/ml, extract from isolate LP_LS026 exhibited 100 % growth inhibition against M. oryzae 33. The most active isolate (LP_LS026) was identified based on their nucleotide sequences of ITS region as Exserohilum sp. (accession number KY024593). The result could be concluded that endophytic fungi isolated from N. lotus exhibited growth inhibition to rice blast fungus and could be a good source of biocontrol agent.
Keywords: endophytic fungi; rice blast disease; Magnaporthe oryzae
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References
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