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This study aims to study the mass balance of chemical oxygen demand (COD), nitrogen (N) and phosphorus (P) in enhanced biological phosphorus removal (EBPR) bioreactor. This study consists of anaerobic-anoxic-aerobic conditions. Acetic acid was used as a carbon source for synthetic wastewater through 60 days of solid retention time (SRT). The rates of TCOD, TN and TP in aerobic condition were 19.60, 1.06 and 1.34 g/d, respectively. They were reduced to be 1.12, 3.52 x 10-2 and 0.41 g/d, respectively, in the effluent. The concentrations of TCOD, TN and TP in effluent were 0.21±0.73, 0.07±0.00 and 8.35±2.34 mg/L. Generally, the EBPR resulted TCOD, TN and TP removal as much as 99.97±0.13, 99.49±0.09 and 61.95.48 %, respectively. Using 60 days of SRT, the efficiency of phosphorus removal can be more than 60 %. The Mass balance can be used as a guideline for controlling the EBPR system and it is possible to apply in municipal wastewater treatment systems.
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