Size-based Determination of Polysaccharides by Taylor Dispersion Analysis with UV-Photochemical Oxidation Detection
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Abstract
Taylor dispersion analysis (TDA) is an analytical method for the determination of the hydrodynamic radius (Rh) or the molecular diffusion coefficient (D) of a solute/compound by analysis from the peak broadening of a plug of the solute/compound under the condition of laminar Poiseuille flow. UV detection mode is commonly employed for TDA, however; the main limitation of TDA-UV method is the lack of a sensitive detection modality for non-UV absorbing solutes/compounds. In this work, we therefore present an alternative detection mode, UV-photochemical oxidation detection (UV-POD), suitable for TDA in order to determine the size of non UV-absorbing polysaccharides. TDA-UV-POD detects the UV-absorbing photooxidized products of polysaccharides in a strong alkaline media (130 mM NaOH) by using the capillary electrophoresis (CE) instrumentation equipped with a UV detector. The irradiation wavelength of 266 nm was selected. Backscattering interferometry (BSI), another kind of suitable detection mode, which measures the relative change of the refractive index (RI) between water eluent and sample, was employed to compare the hydrodynamic radius. Under the conditions of validity of TDA, the mass average Rh values obtained by TDA-UV-POD and TDA-BSI was found in good agreement. Selection of detection mode for TDA is based on the physical properties of the interest compounds and the desired sensitivity in detection.
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Ju, H., Zhang, X. and Wang, J., 2011, Carbohydrate Detection Using Nano structured Biosensing, pp. 393-424, In NanoBiosensing: Principles, Development and Application, Springer, New York.
Gortatowsky, C., 1955, Sugar and other carbohydrates in carbonated beverages: In use of sugars and other carbohydrates in the food industry, J. Amer. Chem Soc. 12: 70-74.
Schoch, T.J. and Elder, A.L., 1955, Starches in the food industry: In use of sugars and other carbohydrates in the food industry, J. Amer. Chem Soc. 12: 21-34.
Burd, A. and Huang, L., 2008, Chapter 11: Carbohydrates and Cutaneous Wound Healing A2 – Garg, Hari G., pp 253-274 In Cowman, M.K. and Hales, C.A. (Eds.), Carbohydrate Chemistry, Biology and Medical Applications, Elsevier, Oxford.
Avila, L.Z., Gianolio, D.A., Konowicz, P.A., Philbrook, M., Santos, M.R. and Miller, R.J., 2008, Chapter 15: Drug Delivery and Medical Applications of Chemically Modified Hyaluronan A2 – Garg, Hari G., pp 333-357, In Cowman, M.K. and Hales, C.A. (Eds.), Carbohydrate Chemistry, Biology and Medical Applications, Elsevier, Oxford.
Striegel, A.M., Isenberg, S.L., Côté and G.L., 2009, An SEC/MALS study of alternan degradation during size-exclusion chromatographic analysis, Anal. Bioanal. Chem. 394: 1887-1893.
Gaborieau, M., Gilbert, R.G., Gray-Weale, A., Hernandez, J.M. and Castignolles, P., 2007, Theory of multiple-detection size-exclusion chromatography of complex branched polymers, Macromol. Theory Simul. 16: 13-28.
Gaborieau, M., Nicolas, J., Save, M., Charleux, B., Vairon, J.P., Gilbert, R.G. and Castignolles, P., 2008, Separation of complex branched polymers by size-exclusion chromatography probed with multiple detection, J. Chromatogr. A 1190: 215-223.
Rolland-Sabaté, A., 2017, High-Perfor mance Size-Exclusion Chromatography coupled with on-line Multi-angle Laser Light Scattering (HPSEC-MALLS), Bentham Science Publishers, Sharjah.
Rolland-Sabaté, A., Battu, S., Bonfils, F., Chelbi, K. and Martin, M., 2017, Field-Flow Fractionation (FFF): In Advances in Physicochemical Properties of Bio polymers (Part 1), Bentham Science Publishers, Sharjah.
Giddings, J.C., 1966, A new separation concept based on a coupling of concentration and flow nonuniformities, Separat. Sci. 1: 123-125.
Messaud, F.A., Sanderson, R.D., Runyon, J.R., Otte, T., Pasch, H. and Williams, S.K.R., 2009, An overview on field-flow fractionation techniques and their applications in the separation and characterization of polymers, Prog. Polym. Sci. 34: 351-368.
Volpi, N., Maccari, F. and Linhardt, R.J., 2008, Capillary electrophoresis of complex natural polysaccharides, Electrophoresis 29: 3095-3106.
Rovio, S., Yli-Kauhaluoma, J. and Siren, H., 2007, Determination of neutral carbohydrates by CZE with direct UV detection, Electrophoresis 28: 3129-3135.
Rovio, S., Simolin, H., Koljonen, K. and Siren, H., 2008, Determination of mono saccharide composition in plant fiber materials by capillary zone electro phoresis, J. Chromatogr. A 1185: 139-144.
Sarazin, C., Delaunay, N., Costanza, C., Eudes, V., Mallet, J.M. and Gareil, P., 2011, New avenue for mid-UV-range detection of underivatized carbohydrates and amino acids in capillary electrophoresis, Anal. Chem. 83: 7381-7387.
Oliver, J.D., Gaborieau, M., Hilder, E.F. and Castignolles, P., 2013, Simple and robust determination of monosaccharides in plant fibers in complex mixtures by capillary electrophoresis and high performance liquid chromatography, J. Chromatogr. A 1291: 179-186.
Schmid, T., Himmelsbach, M., Oliver, J.D., Gaborieau, M., Castignolles, P. and Buchberger, W., 2015, Investigation of photochemical reactions of saccharides during direct ultraviolet absorbance detection in capillary electrophoresis, J. Chromatogr. A 1388: 259-266.
Schmid, T., Himmelsbach, M. and Buchberger, W.W., 2016, Investigation of photochemical reaction products of glucose formed during direct UV detection in CE, Electrophoresis 37: 947-953.
Oliver, J.D., Rosser, A.A., Fellows, C.M., Guillaneuf, Y., Clement, J.L., Gaborieau, M. and Castignolles, P., 2014, Understanding and improving direct UV detection of monosaccharides and disaccharides in free solution capillary electrophoresis, Anal. Chim. Acta 809: 183-193.
Sarazin, C., Delaunay, N., Costanza, C., Eudes, V. and Gareil, P., 2012, Application of a new capillary electrophoretic method for the determination of carbohydrates in forensic, pharmaceutical, and beverage samples, Talanta 99: 202-206.
Sarazin, C., Delaunay, N., Costanza, C., Eudes, V. and Gareil, P., 2013, On the use of capillary electrophoresis for the determination of inorganic anions and cations, and carbohydrates in residues collected after a simulated suicide bombing attack, Talanta 103: 301-305.
Toutounji, M.R., van Leeuwen, M.P., Oliver, J.D., Shrestha, A.K., Castignolles, P. and Gaborieau, M., 2015, Quantification of sugars in breakfast cereals using capillary electrophoresis, Carbohydr. Res. 408: 34-141.
Alinat, E., Jemmali, S., Delaunay, N., Archer, X. and Gareil, P., 2015, Analysis of underivatized cellodextrin oligosaccha rides by capillary electrophoresis with direct photochemically induced UV-detection, Electrophoresis 36: 1555-1563.
Saetear, P., Chamieh, J., Kammer, M.N., Manuel, T.J., Biron†, J.P., Bornhop, D.J. and Cotte, H., 2017, Taylor Dispersion Analysis of polysaccharides using backscattering interferometry, Anal. Chem. 89: 6710-6718.
Chamieh, J. and Cottet, H., 2012, Comparison of single and double detection points Taylor dispersion analysis for monodisperse and polydisperse samples, J. Chromatogr. A 1241: 123-127.
Provencher, S.W., 1982, A constrained regularization method for inverting data represented by linear algebraic or integral equations, Comput. Phys. Commun. 27: 213-227.
Cottet, H., Biron, J.P. and Martin, M., 2007, Taylor dispersion analysis of mixtures, Anal. Chem. 79: 9066-9073.
Cottet, H., Biron, J.P. and Martin, M., 2014, On the optimization of operating conditions for Taylor dispersion analysis of mixtures, Analyst 139: 3552-3562.
Rolland-Sabaté, A., Guilois, S., Jaillais, B. and Colonna, P., 2011, Molecular size and mass distributions of native starches using complementary separation methods: Asymmetrical flow field flow fractionation (A4F) and hydrodynamic and size exclusion chromatography (HDC-SEC), Anal. Bioanal. Chem. 399: 1493-1505.
Rolland-Sabaté, A., Mendez-Montealvo, M.G., Colonna, P. and Planchot, V., 2008, Online determination of structural properties and observation of deviations from power law behavior, Biomacro molecules 9: 1719-1730.