Size-based Determination of Polysaccharides by Taylor Dispersion Analysis with UV-Photochemical Oxidation Detection

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พูนทวี แซ่เตีย
Laurent Leclercq
Hervé Cottet

Abstract

Taylor dispersion analysis (TDA) is an analytical method for the determination of the hydrodynamic radius (Rh) or the molecular diffusion coefficient (D) of a solute/compound by analysis from the peak broadening of a plug of the solute/compound under the condition of laminar Poiseuille flow. UV detection mode is commonly employed for TDA, however; the main limitation of TDA-UV method is the lack of a sensitive detection modality for non-UV absorbing solutes/compounds. In this work, we therefore present an alternative detection mode, UV-photochemical oxidation detection (UV-POD), suitable for TDA in order to determine the size of non UV-absorbing polysaccharides. TDA-UV-POD detects the UV-absorbing photooxidized products of polysaccharides in a strong alkaline media (130 mM NaOH) by using the capillary electrophoresis (CE) instrumentation equipped with a UV detector. The irradiation wavelength of 266 nm was selected. Backscattering interferometry (BSI), another kind of suitable detection mode, which measures the relative change of the refractive index (RI) between water eluent and sample, was employed to compare the hydrodynamic radius. Under the conditions of validity of TDA, the mass average Rh values obtained by TDA-UV-POD and TDA-BSI was found in good agreement. Selection of detection mode for TDA is based on the physical properties of the interest compounds and the desired sensitivity in detection.

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วิทยาศาสตร์กายภาพ
Author Biographies

พูนทวี แซ่เตีย, ภาควิชาเคมี คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล

ภาควิชาเคมี คณะวิทยาศาสตร์ มหาวิทยาลัยมหิดล ถนนพระรามที่ 6 แขวงทุ่งพญาไท เขตราชเทวี กรุงเทพมหานคร 10400

Laurent Leclercq

IBMM, Université de Montpellier, CNRS, ENSCM, Montpellier 34095

Hervé Cottet

IBMM, Université de Montpellier, CNRS, ENSCM, Montpellier 34095

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