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Ink is a by-product of the squid processing that comes from household consumption or the seafood industry. The disposal of ink seems to cause environmental pollution. This research aimed to study the fundamental information and the application of cephalopod ink in 3 species, i.e. Bigfin reef squid (Sepioteuthis lessoniana), Pharaoh cuttlefish (Sepia pharaonis), and Marbled octopus (Amphioctopus aegina). The results showed that the moisture contents of those inks were 87.82±1.66, 85.08±0.70 and 85.57±0.12 %, and the pH values were 11, 8 and 10 in Bigfin reef squid, Pharaoh cuttlefish and Marbled octopus, respectively. The highest amount of protein content was from Bigfin reef squid ink (144.15±5.48 mg/mL), followed by Pharaoh cuttlefish ink (136.24±0.60 mg/mL) and Marbled octopus ink (104.17±0.68 mg/mL), which was significantly different at p < 0.05. The main color composition in the ink was predicted as melanin that revealed the maximum absorption (λmax) at 307 nm. Ink jelly was prepared for making a high value application. The proximate analysis of the ink jelly was shown 81.87-83.89 % moisture content, 1.52-1.92 % ash, 0.04-0.05 % crude protein, 0.02 % crude fat, and 14.18-16.06 % carbohydrate content. The colorimetric analysis showed the highest value of lightness, redness, yellowness and chroma in Pharaoh cuttlefish ink jelly and the highest value of hue angle in Marbled octopus ink jelly.
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