ผลของสารควบคุมการเจริญเติบโตต่อการเพิ่มจำนวนยอดและการชักนำให้เกิดรากของกะเพราแดงในสภาพปลอดเชื้อ
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Abstract
Ocimum sanctum L. is a medicinal plant that contains high antioxidant activity. Under natural conditions, genetic variation always occurs because of cross-pollination among genus. However, plant tissue culture can provide a number of plants with genetic stability. Therefore, the objective of this study was to investigate the effect of plant growth regulators on shoot multiplication from single node explants and root induction of O. sanctum under aseptic conditions. For shoot multiplication, single node explants were cultured on MS (Murashige and Skoog) medium supplemented with 0, 0.5, 1.0, 1.5 and 2.0 mg/L BA (6-benzyladenine) in combination with 0 and 0.5 mg/L IAA (indole-3-acetic acid) or NAA (1-naphthylacetic acid) for 4 weeks. The results showed that 100 % shoot proliferation occurred on all tested media. However, supplementation with IAA and NAA resulted in callus formation at the base of the shoot. The number of shoots, shoot length and number of nodes per shoot obtained on MS medium supplemented with 0.5 mg/L BA of 3.00±0.43 shoots, 0.71±0.24 cm and 1.81±0.26 nodes, respectively. For root induction, the shoots cultured on ½ MS or MS medium supplemented with 0 1.0 or 1.5 mg/L NAA and IBA (indole-3-butyric acid) for 4 weeks performed 100 % of root formation but NAA also resulted in callus at the base of the shoot. The number of roots and root length obtained on ½ MS medium of 12.2±4.20 roots and 2.08±0.29 cm, respectively.
Keywords: Ocimum sanctum L.; shoot multiplication; root induction; cytokinin; auxin
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References
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