Effect of Plant Growth Regulators on Diterpene Lactone Production in Callus Culture of Andrographis paniculata
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Abstract
Andrographis paniculata is a medicinal plant which attributes for diverse pharmacological properties. The main secondary metabolite of A. paniculata is total lactones which comprise of 4 compounds, such as andrographolide, neoandrographolide, 14-deoxy-11,12-didehydroandrogra pholide and 14-deoxyandrographolide. This research aims to induce callus for secondary metabolite production by cell culture technique. The effect of growth regulators, i.e. cytokinins as benzylaminopurine (BAP) and auxins as 2,4-dichlorophenoxyacetic acid (2,4-D), on the induction of callus from explants cultured on Murashige and Skoog (MS) was studied. From the result, MS supplemented with BAP and 2,4-D at concentrations of 1 and 1 mg/L, respectively, can induce callus formation. Then compounds in the callus extract were analyzed by the TLC techniques, and lactones were determined by colorimetric method using UV-Vis spectrophotometer. The result showed that Andrographis paniculata callus contains 7.31±0.15 %w/w total lactones.
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