Effect of Medium, Type and Concentration of Sugar on In vitro Medium-Term Storage of Musa (ABB Group) ‘Namwa Pakchong 50’
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Effects of basal media, type and concentration of sugar on medium-term storage of Musa (ABB group) ‘Namwa Pakchong 50’. The experiment 1 was arranged in completely randomized design with 2 treatments. Shoots were cultured on MS and ½MS medium for four months. The result showed that there was not significantly different in survival percentage between these two media. The survival percentages of MS and ½MS were 95 and 92.5, respectively. After that the plantlets of each treatments were recovered on MS medium supplemented with 5 mg L-1 BA for four weeks. We found that plantlets from both treatments had survival percentage of 97.3. Then ½MS medium was chosen for the second experiment which was designed with 6 treatments. Shoots were cultured on ½MS medium supplemented with sucrose at the concentrations of 30 (control), 60 and 90 g L-1 and mannitol at the concentrations of 30, 40 and 50 g L-1 for four months. The result showed that the survival percentages of shoots cultured on ½MS medium supplemented with sucrose at all concentrations were 90-92.5. In contrast, mannitol gave 0-40 % survival. The plantlets were recovered on the same medium as the first experiment. The result showed that the shoots cultured on ½MS medium supplemented with various concentrations of mannitol were dead, while the other cultured on ½MS medium supplemented with sucrose at all concentrations gave survival rate at 94-97 %. Moreover, the shoots cultured on ½MS medium supplemented with 90 g L-1 sucrose had the highest shoot circumference (3.66 cm) and significantly different compared with the others.
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