Effect of Medium, Type and Concentration of Sugar on In vitro Medium-Term Storage of Musa (ABB Group) ‘Namwa Pakchong 50’

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น้ำเพชร พรหมสุวรรณ์
ทัศไนย จารุวัฒนพันธ์
อารยา อาจเจริญ เทียนหอม

Abstract

Effects of basal media, type and concentration of sugar on medium-term storage of Musa (ABB group) ‘Namwa Pakchong 50’. The experiment 1 was arranged in completely randomized design with 2 treatments. Shoots were cultured on MS and ½MS medium for four months. The result showed that there was not significantly different in survival percentage between these two media. The survival percentages of MS and ½MS were 95 and 92.5, respectively.  After that the plantlets of each treatments were recovered on MS medium supplemented with 5 mg L-1 BA for four weeks. We found that plantlets from both treatments had survival percentage of 97.3. Then ½MS medium was chosen for the second experiment which was designed with 6 treatments. Shoots were cultured on ½MS medium supplemented with sucrose at the concentrations of 30 (control), 60 and 90 g L-1 and mannitol at the concentrations of 30, 40 and 50 g L-1 for four months. The result showed that the survival percentages of shoots cultured on ½MS medium supplemented with sucrose at all concentrations were 90-92.5. In contrast, mannitol gave 0-40 % survival. The plantlets were recovered on the same medium as the first experiment. The result showed that the shoots cultured on ½MS medium supplemented with various concentrations of mannitol were dead, while the other cultured on ½MS medium supplemented with sucrose at all concentrations gave survival rate at 94-97 %. Moreover, the shoots cultured on ½MS medium supplemented with 90 g L-1 sucrose had the highest shoot circumference (3.66 cm) and significantly different compared with the others.

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วิทยาศาสตร์ชีวภาพ
Author Biographies

น้ำเพชร พรหมสุวรรณ์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

ทัศไนย จารุวัฒนพันธ์

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

อารยา อาจเจริญ เทียนหอม

ภาควิชาพืชสวน คณะเกษตร มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตบางเขน แขวงลาดยาว เขตจตุจักร กรุงเทพมหานคร 10900

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