Appropriate Extraction Methods and Conditions of Antioxidant Compounds in Longan

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สุกัญญา เขียวสะอาด
อัศวิน ดาดูเคล
อาทิตย์ ยาวุฑฒิ
พิสิษฐ์ วิมลธนสิทธิ์

Abstract

The aim of this research was to study appropriate extraction methods and conditions of antioxidant compounds from longan seed by comparing 3 methods; i.e. maceration, reflux and magneto pulse extraction. The different extraction times were studies at 3, 5 and 7 days for maceration, 1, 3 and 6 hours for reflux and 15, 30 and 45 minutes for pulsed electric field extraction. The extracts were evaluated for their antioxidant activity and total phenolic contents. The reflux extract for 6 hours showed the highest total phenolic content and antioxidant activity, followed by the reflux extract for 3 hours and the pulsed electric field extract for 45 minutes, respectively. Therefore, the reflux extraction for 6 hours is the suitable method and condition for antioxidant compounds extraction from longan seed. This condition gave the total phenolic content of 271.33±6.11 mg GAE/g dry extract and possessed the antioxidant activity by DPPH assays with the IC50 value of 36.9±0.4 μg/mL; FRAP value of 1,010.42±8.59 μmol FeSO4/g dry extract.

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วิทยาศาสตร์ชีวภาพ
Author Biographies

สุกัญญา เขียวสะอาด

คณะเภสัชศาสตร์ มหาวิทยาลัยพายัพ ตำบลหนองป่าครั่ง อำเภอเมือง จังหวัดเชียงใหม่ 50000

อัศวิน ดาดูเคล

คณะเภสัชศาสตร์ มหาวิทยาลัยพายัพ ตำบลหนองป่าครั่ง อำเภอเมือง จังหวัดเชียงใหม่ 50000

อาทิตย์ ยาวุฑฒิ

คณะวิศวกรรมศาสตร์ มหาวิทยาลัยเทคโนโลยีราชมงคลล้านนา ถนนห้วยแก้ว ตำบลช้างเผือก อำเภอเมือง จังหวัดเชียงใหม่ 50300

พิสิษฐ์ วิมลธนสิทธิ์

คณะวิศวกรรมศาสตร์ มหาวิทยาลัยเทคโนโลยีราชมงคลล้านนา ถนนห้วยแก้ว ตำบลช้างเผือก อำเภอเมือง จังหวัดเชียงใหม่ 50300

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