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Excessive fructose consumption promotes oxidative stress leading to liver damage and the development of NALFD (non-alcoholic fatty liver disease). Naringin, a flavanone glycoside found in citrus fruit, has antioxidant and hypolipidemic properties. Therefore, the aim of this study was to investigate the effect of naringin on NADPH oxidase expression, oxidative damage, and histopathological changes in liver of fructose-fed rats. Male Sprague- Dawley rats were given 10 % (w/v) fructose in drinking water for 12 weeks. Naringin (100 mg/kg/day) was administered orally to the rats for the last 4 weeks of fructose overload. After 12 weeks of treatment, liver histopathological changes were observed with haematoxylin and eosin staining. Liver protein expression of NADPH oxidase subunits (NOX4 and p47phox) and 4-hydroxynonenal (4HNE) were determined by Western blot analysis. Results showed that consumption of fructose solution increased the liver expressions of p47 phox, NOX4, and 4-HNE. Administration of naringin ameliorated these alterations. Histological observations revealed the enlarged liver cells and lipid droplets in the liver cytosol of fructose-fed rats. Treatment of the fructose-fed rats with naringin improved these histological changes. These results demonstrate that oral treatment with naringin could reduce liver oxidative stress by down-regulating NADPH oxidase subunits expression, and ameliorating histopathological alterations in liver of fructose-fed rats. Naringin may be a potential therapeutic strategy for the attenuation of fructose-induced liver damages.
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