Efficacy of Endophytic Fungi Isolated from Rice Plants against Rhizoctonia solani, Causing of Rice Sheath Blight Disease
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Abstract
This research aimed to evaluate the efficacy of endophytic fungi to control rice sheath blight caused by Rhizoctonia solani. The in vitro study, 102 endophytic fungi, which were isolated from leaves and stems of rice and preserved in the Division of Microbiology, Phranakhon Si Ayutthaya Rajabhat University, were screened for antifungal activity against R. solani according to the dual culture method. The inhibition zone was exhibited from the three isolates, namely AT18-1, AT19-3, and AY31, under incubation at 30 ºC for 7 days. The percentages of growth inhibition were 31.61, 33.33, and 31.11 %, respectively. The sequence analysis of these three isolates was performed at the internal transcribed spacer (ITS) region and they were identified as Penicillium oxalicum. The in vivo antagonistic was done in the greenhouse condition by infection of R. solani on the stem of RD47 rice variety. The results showed that completely inhibited disease by endophytic fungi was observed after 21 days, whereas the negative control was still observed rice sheath blight disease.
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