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This research aimed to evaluate the efficacy of endophytic fungi to control rice sheath blight caused by Rhizoctonia solani. The in vitro study, 102 endophytic fungi, which were isolated from leaves and stems of rice and preserved in the Division of Microbiology, Phranakhon Si Ayutthaya Rajabhat University, were screened for antifungal activity against R. solani according to the dual culture method. The inhibition zone was exhibited from the three isolates, namely AT18-1, AT19-3, and AY31, under incubation at 30 ºC for 7 days. The percentages of growth inhibition were 31.61, 33.33, and 31.11 %, respectively. The sequence analysis of these three isolates was performed at the internal transcribed spacer (ITS) region and they were identified as Penicillium oxalicum. The in vivo antagonistic was done in the greenhouse condition by infection of R. solani on the stem of RD47 rice variety. The results showed that completely inhibited disease by endophytic fungi was observed after 21 days, whereas the negative control was still observed rice sheath blight disease.
Bureau of Rice Policy and Strategy, Rice department, Demand and Supply Thai rice, Year 2020-2021, Available Source: http://www.ricethailand.go.th/web/images/manual_rice63-17-3-63.pdf, May 8, 2020. (in Thai)
Rice Department, Ministry of Agriculture and Cooperatives, Rice Knowledge Bank Available Source: http://www.ricethai land.go.th/rkb, June 27, 2020. (in Thai)
Ou, S.H., 1985, Rice Diseases, 2nd Ed., Commonwealth Mycological Institute, Kew, Surrey, 380 p.
Teng, P.S., Torres, C.Q., Nuque, F.L. and Calvero, S.B., 1990, Current Knowledge on Crop Losses in Tropical Rice, pp. 39-53, In IRRI, Crop Loss Assessment in Rice, International Rice Research institute, Manila.
Savary, S., Willocquet, L., Elazegui, F.A., Teng, P.S., Du, V., Zhu, D., Tang, S., Huang, X., Lin, H., Singh, M. and Srivastava, R.K., 2000, Rice pest constraints in tropical Asia: Characterization of injury profiles in relation to production situations, Plant Dis. 84: 341-356.
Thapanapongworakul, P. and Saimongkol, S., 2015, Efficiency of antagonistic bacterium Bacillus megaterium strain No. 16 for controlling sheath blight disease of RD6 rice variety, J. Agric. 31(3): 301-310. (in Thai)
Kaewkrajay, C., Dethoup, T. and Sathitpanawong, L., 2014, Efficacy of endophytic fungi isolated from Sesbania javanica against plant pathogenic fungi, Khon Kaen Agric. J. 42(3): 271-282. (in Thai)
Latz, M.A.C., Jensen, B., Collinge, D.B. and Jorgensen H.J.L., 2018, Endophytic fungi as biocontrol agents: Elucidating mechanisms in disease suppression, Plant Ecol. Divers. 11: 555-567.
Jitsuwannarak, K. and Wongcharoen, A., 2016, Efficiency of endophytic fungi to control rice blast disease (Oryza sativa L.), Khon Kaen Agric. J. 44(Suppl. 1): 232-237. (in Thai)
Pripdeevech, P., Tanapichatsakul, C., Chimthai, S., Phormmard, W. and Brooks, S., 2018, Antagonistic effect of endophytic fungal, Fusarium sp. MFLUCC16-1462, against Magnaporthe oryzae, Thai J. Sci. Technol. 7(Suppl. 6): 581-591.
Park, Y.H., Chung, J.Y., Ahn, D.J., Kwon, T.R., Lee, S.K., Bae, I., Yun, H.K. and Bae, H., 2015, Screening and characterization of endophytic fungi of Panax ginseng Meyer for biocontrol activity against ginseng pathogens, Biol. Control 91: 71-81.
Strobel, G.A., 2006, Muscodor albus and its biological promise, J. Ind. Microbiol. Biotechnol. 33: 514-522.
Manoch, L., Athipunyakom, P., Jaroenthai, K., Busarakam, K., Jeamjitt, O., Dethoup, T., Kokaew, J. and Poochinya, P., 2004, Plant parasitic fungi on fruits & vetget ables and soil fungi from termite mounds, pp. 544-552, In Proceedings of 42nd Kasetsart University Annual Conference: Plants, Kasetsart University, Bangkok.
George, M.L.C., Nelson, R.J., Zeigler, R.S. and Leung, H., 1998, Rapid population analysis of Magnaporthe grisea by using rep-PCR and endogenous repetitive DNA sequences, Phytopathology 88: 223-229.
Kumar, K.V.K., Yellareddygari, S.K.R., Reddy, M.S., Kloepper, J.W., Lawrence, K.S., Zhou, X.G., Sudini, H., Groth, D.E., Raju, S.K. and Miller, M.E., 2012, Efficacy of Bacillus subtilis MBI 600 against sheath blight caused by Rhizoctonia solani and on growth and yield of rice, Rice Sci. 19: 55-63.
Anh, S.W., Pena, R.L., Candole, B.L. and Mew, T.W., 1986, A new scale for rice sheath blight disease assessment, IRRI 11(6): 17.
Yu, J., Wu, Y., He, Z., Li, M., Zhu, K. and Gao, B., 2018, Diversity and antifungal activity of endophytic fungi associated with Camellia oleifera, Mycobiology 46: 85-91.
Chowdhary, K. and Kaushik, N., 2018, Biodiversity study and potential of fungal endophytes of peppermint and effect of their extract on chickpea rot pathogens, Arch. Phytopathol. Plant Protect. 51: 139-155.
Ibrahim, M., Kaushik, N., Sowemimo, A., Chhipa, H., Koekemoer, T., van de Venter M. and Odukoya, O.A., 2017, Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa, Pharm. Biol. 55: 590-595.
Luo, Z.P., Lin, H.Y., Ding, W.B., He, H.L. and Li, Y.Z., 2015, Phylogenetic diversity and antifungal activity of endophytic fungi associated with Tephrosia purpurea, Mycobiology 43: 435-443.
Paul, N.C., Kim, W.K., Woo, S.K., Park, M.S. and Yu, S.H., 2006, Diversity of endophytic fungi associated with Taraxacum coreanum and their antifungal activity, Mycobiology 34: 185-190.
Martinez-Beringola, M.L., Salto, T., Vázquez, G., Larena, I., Melgarejo, P. and de Cal, A., 2013, Penicillium oxalicum reduces the number of cysts and juveniles of potato cyst nematodes, J. Appl. Microbiol. 115: 199-206.
Ahmad, A., Shafique, Shafique, S. and Akram, W., 2014, Penicillium oxalicum directed systemic resistance in tomato against Alternaria alternata, Acta Physiol. Plant 36: 1231-1240.
Sabuquillo, P., Cal, A.D. and Melgarejo, P., 2006, Biocontrol of tomato wilt by Penicillium oxalicum formulations in different crop conditions, Biol. Control 37: 256-265.
Sempere, F. and Santamarina, M.P., 2010, Study of the interactions between Penicillium oxalicum Currie & Thom and Alternaria alternata (Fr.) Keissler. Braz., J. Microbiol. 41: 700-706.
Ren, Y., Chao, L.H., Sun, J., Chen X.N., Yao, H.N., Zhu, Z.X., Dong, D., Liu, T., Tu, P.F. and Li, J., 2019, Two new polyketides from the fungus Penicillium oxalicum MHZ153, Nat. Prod. Res. 33: 347-353.
Li, Q.Q., Dang, L.Z., Zhang, Y.P., Jiang, J.X., Zhang, C.M., Xiang, N.J., Yang, H.Y., Du, G. and Duan, Y.Q., 2015, Isocoumarins from the fermentation products of a plant entophytic fungus Penicillium oxalicum, J. Asian Nat. Prod. Res. 17: 876-881.
Li, X., Li, X.M., Zhang, P., Wang, B.G., 2015, A new phenolic enamide and a new meroterpenoid from marine alga-derived endophytic fungus Penicillium oxalicum EN-290, J. Asian Nat. Prod. Res. 17: 1204-1212.
Zhang, P., Li, X.M., Liu, H., Li, X. and Wang, B.G., 2015, Two new alkaloids from Penicillium oxalicum EN-201, an endophytic fungus derived from the marine mangrove plant Rhizophora stylosa, Phytochem. Lett. 13: 160-164.
Yuan, L., Huang, W.Z., Zhou K., Wang, Y.D., Dong, W., Du, G., Gao, X.M., Ma, Y.H. and Hu, Q.F., 2015, Butyrolactones derivatives from the fermentation products of a plant endophytic fungus Penicillium oxalicum,
Nat. Prod. Res. 29: 1914-1919.
Wongcharoen, A., 2014, Screening of endophytic fungi from rice (Oryza sativa L.) against rice pathogenic fungi, Khon Kaen Agric. J. 42(3): 385-396. (in Thai)
Guo, T. and Liao, M., 2014, Suppression of Rhizoctonia solani and induction of host plant resistance by Paenibacillus kribbensis PS04 towards controlling of rice sheath blight, Biocontrol Sci. Technol. 24: 116-121.
Chamswarng, C., Intanoo, W. and Noisai, B., 2018, Efficacy of biological products of Bacillus siamensis RRK1-Rif for reducing sheath blight and dirty panicle of rice, Agric. Sci. J. 49: 1-14.
Chen, L.H., Zhang, J., Shao, X.H., Wang, S.S., Miao, Q.S., Mao, X.Y., Zhai, Y.M. and She, D.L., 2015, Development and evaluation of Trichoderma asperellum preparation for control of sheath blight of rice (Oryza sativa L.) Biocontrol Sci. Technol. 25: 316-328.