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This study aimed to assess growth, biomass production, and biochemical compounds of a cyanobacterial strain NUACC 2.57 isolated from Mae La River, Sing Buri. The results obtained from morphological examinations such as terminal heterocyst cells. Together with the phylogenetic relationship based on the partial 16S rDNA, it was shown that the studied strain belongs to the genus Anabaenopsis. In this study, the partial 16S rDNA sequences could not identify a strain NUACC2.57 at the species level; however, the tested strain could be identified as A. elenkinii according to the morphological comparison based on the shape and cell dimensions of cell and trichome. Then, the effects of nitrogen concentrations on growth and biochemical compounds of a strain NUACC2.57 were studied. The strain was cultured in BG medium with three different nitrogen concentrations (0.0, 1.5, and 3.0 g/L). Each treatment was run in triplicate for 28 days. The results exhibited that the strain could grow in all nitrogen concentrations tested. Moreover, the maximum values of biomass (2.48±0.07 g DW/L), phycocyanin (68.27±11.90 mg/g DW), total protein (537.07±8.36 mg/g DW), and carbohydrate (39.55±8.19 mg/g DW) contents were obtained significantly (p < 0.05) from the strain NUACC 2.57 cultured in BG medium without nitrogen (0 g/L). Considering the results obtained in the present study, the cyanobacterium A. elenkinii NUACC2.57 could be utilized for production of high-value compounds such as protein and phycocyanin. Moreover, the cultivation of A. elenkinii NUACC2.57 without nitrogen also could reduce the cost of nutrient.
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