Main Article Content
Centella asiatica (L.) Urban is an important bioactive compound enriched medicinal herb, which have been shown to potentially prevent non-communicable diseases. Therefore, the objective of this study was to determine contents of triterpene, phenolic compound and antioxidant capacity of six accessions of C. asiatica. The experiment was conducted as a completely randomized design (CRD) with three replications. The results indicated that the contents of triterpene, phenolic compound and antioxidant capacity were significantly different (p ≤ 0.05). TU-CA 12 contained the greatest contents of madecassoside, asiaticoside, caffeic acid, DPPH and ABTS radical scavenging capacities. The highest p-coumaric acid and total phenolic contents were found in TU-CA 5 and TU-CA 13, respectively. In addition, total phenolic content and caffeic acid were positively correlated with antioxidant capacity and exhibited their antioxidant capacities higher than triterpenes. Therefore, the results of this study should be used to select the accession of C. asiatica with high antioxidant contents and its antiradical capacities.
 Park, J.H., Choi, J.Y., Son, D.J., Park, E.K., Song, M.J., Hellström M. and Hong, J.T., 2017, Anti-inflammatory effect of titrated extract of Centella asiatica in phthalic anhydride-induced allergic dermatitis animal model, Int. J. Mol. Sci. 18: 1-14.
 Somboonwong, J., Kankaisre, M., Tantisira, B. and Tantisira, M.H, 2012, Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: experimental animal study, BMC Complement Altern. Med. 12: 1-7.
 Veerendra, K.M.H. and Gupta, Y.K., 2002, Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats, J. Ethnopharmacol. 79: 253-260.
 Ramesh, B.N., Girish, T.K., Raghavendra, R.H., Akhilender Naidu, K., Akhilender Naidu, U.J.S. and Rao, K.S., 2014, Comparative study on anti-oxidant and anti-inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts, J. Pharm. Bioallied Sci. 6: 86-91.
 Tomas-Barberan, F.A. and Espin, J.C., 2001, Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables, J. Sci. Food Agric. 81: 853-876.
 Randriamampionona, D., Diallo, B, Rakotoniriana, F., Rabemanantsoa, C., Cheuk, K, Corbisier, A.M., Mahillon, J., Ratsimamanga, S. and El Jaziri, M., 2007, Comparative analysis of active constituents in Centella asiatica samples from Madagascar: application for ex situ conservation and clonal propagation, Fitoterapia 78: 482-489.
 Alqahtani, A., Cho, J.L., Wong, K.H., Li, K., Razmovski-Naumovski, V. and Li, G.Q., 2017, Differentiation of three Centella species in Australia as inferred from morphological characteristics, ISSR molecular fingerprinting and phytochemical composition, Front. Plant Sci. 8: 1-16.
 Somwong, P., 2006, Isolation, Purification and Quantitative Determination of Asiaticoside, Madecassoside, Asiatic Acid and Madecassic Acid in Varieties of Centella Asiatica (L.) URB., Master Thesis, Chulalongkorn University, Bangkok, 130 p.
 Fatema, S.U., 2006, Effect of Fertilizers, Planting Time and Harvesting Period on the Yield of Thankuni (Centella asiatica) under 80 % Photosynthetically Active Radiation, Master Thesis, Bangladesh Agricultural University, Bangladesh, 49 p.
 Xiao, Z., Lester, G.E., Luo, Y. and Wang, Q., 2012, Assessment of vitamin and carotenoid concentrations of emerging food products: edible microgreens, J. Agric. Food Chem. 60: 7644-7651.
 Jaiarree, N., 2010, Biological Activities of Dioscorea birmanica Prain and Burkill Extract and Its Active Ingredients, Doctoral Dissertation, Thammasat University, Pathum Thani, 130 p.
 Hengjumrut, P., Anukunwithaya, T., Tantisira, M.H., Tantisira, B. and Khemawoot, P., 2017, Comparative pharmacokinetics between adecassoside and asiaticoside presented in a standardised extract of Centella asiatica, ECa 233 and their respective pure compound given separately in rats, Xenobiotica, Early Online 2017: 1-10.
 Oniszcuk, A. and Olech, M., 2016, Optimization of ultrasound-assisted extraction and LC-ESI-MS/MS analysis of phenolic acids from Brassica oleracea L. var. sabellica, Ind. Crop Prod. 83: 359-363.
 Folin, O., and Ciocalteu, V., 1927. On tyrosine and tryptophan determinations in proteins, J. Biol. Chem. 73: 627-650.
 Brand-Williams, W., Cuvelier, M.E. and Berset, C., 1995, Use of a free radical method to evaluate antioxidant activity, Lebenson Wiss Technol. 28: 25-30.
 Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C., 1999, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Rad. Biol. Med. 26: 1231-1237.
 Upadhya, V., Pai, S.R., Sharma, A.K., Hegde, H.V., Kholkute, S.D. and Joshi, R.K., 2014, Compound specific extraction of camptothecin from Nothapodytes nimmoniana and piperine from Piper nigrum using accelerated solvent extractor, J. Anal. Methods Chem. 2014: 1-7.
 Tambunan, A.P., Bahtiar, A. and Tjandrawinata, R.R., 2017, Influence of extraction parameters on the yield, phytochemical, TLC-densitometric quantification of quercetin, and LC-MS profile, and how to standardize different batches for long term from Ageratum conyoides L. leaves, Pharmacogn. J. 9: 767-774.
 Prasad, A., Yadav, K.S., Yadav, N.P., Mathur, A., Sreedhar, R.V., Lal, R.K. and Mathur, A.K., 2016, Biomass and centellosides production in two elite Centella asiatica germplasms from India in response to seasonal variation, Ind. Crops Prod. 94: 711-720.
 Puttarak, P. and Panichayupakaranant, P., 2012, Factors affecting the content of pentacyclic triterpenes in Centella asiatica raw materials, Pharm. Biol. 50: 1508-1512.
 Srivastava, S., Verma, S., Gupta, A., Rajan, S. and Rawat, A.K.S., 2014, Studies on chemotypic variation in Centella asiatica (L.) Urban from nilgiri range of India, JPC J. Planar Chormat. 27: 454-459.
 Orhan, I.E., 2012, Centella asiatica (L.) Urban: From traditional medicine to modern medicine with neuroprotective potential, Evid. Based Complement. Alternat. Med. 2012: 1-8.
 Ramesh, B.N., Girish, T.K., Raghavendra, R.H., Naidu, K.A., Prasada Rao, U.J.S. and Rao, K.S., 2014, Comparative study on anti-oxidant and anti-inflammatory activities of Caesalpinia crista and Centella asiatica leaf extracts, J. Pharm. Bioallied Sci. 6: 86-91.
 Khojah, E.Y., 2010, Development of a GC-MS Method and Analysis of Phenolic Acids in Fruit and Spice Matrices and Evaluation of their Antioxidant Capacity, Doctoral Dissertation, The University of New South Wales, Sydney, 230 p.
 Ferguson, L.R, Shuo-tun, Z. and Harris, P.J., 2005, Antioxidant and antigenotoxic effects of plant cell wall hydroxycinnamic acids in cultured HT-29 cells, Mol. Nutr. Food Res. 49: 585-93.
 Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M. and Merillon, J.M., 2009, Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays, J. Agric. Food Chem. 57: 1768-1774.
 Lopes, G.B., Schulman, H.M. and Lima, M.H., 1999, Polyphenol tannic acid inhibition hydroxyl radical formation from Fenton reaction by complexing ferrous ions, Biochim. Biophys. Acta 1426: 475-482.
 Maulidiani, F., Khatib, A., Shaari, K. and Lajis, N.H., 2014, Chemical characteriza tion and antioxidant activity of three medicinal Apiaceae species, Ind. Crops Prod. 55: 238-247.
 Suzanna, E., 2014, Analysis of Phenolics from Centella asiatica and Verrnonia amygdalina and their Role as Antibacterial and Antioxidant Compounds, Master Thesis, University of Malaya, Kuala Lumpur, 248 p.
 Duh, P.D. and Yen, G.C., 1997, Antioxidant activity of three herbal water extracts, Food Chem. 60: 639-645.
 Günther, B. and Wagner, H., 1996, Quantitative determination of triterpenes in extracts and phytopreparations of Centella asiatica (L.) Urban, Phytomedi cine 3: 59-65.
 Niamnuy, C., Charoenchaitrakool, M., Mayachiew, P. and Devahastin, S., 2013, Bioactive compounds and bioactivities of Centella asiatica (L.) Urban prepared by different drying methods and conditions, Dry Technol. 31: 2007-2015.
 Oliveira, S.D., Souza, G.A.D., Eckert, C.R., Silva, T.A., Sobral, E.S., Fávero, O.A., Ferreirall, M.J.P., Romoffll, P. and Baaderl, W.J., 2014, Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts, Quím. Nova 37: 497-503.
 Kundu, S., Haque, S.M. and Ghosh, B., 2015, Comparative analysis of bioactive compounds in different habitat of Centella asiatica (L.) Urban: Application for in vitro clonal propagation of elite ecotype, J. Appl. Pharm. Sci. 5: 30-36.