อิทธิพลของ ZnSO4 ต่อการเจริญเติบโตและสารต้านอนุมูลอิสระในไมโครกรีนผักขี้หูด ผักชีลาว และแมงลัก
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ธ.ค. 29, 2019
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ZnSO4 สารประกอบฟีนอลิค ฟลาโวนอยด์ ไมโครกรีน
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บทคัดย่อ
ศึกษาผลของ zinc sulfate (ZnSO4) ต่อการเจริญเติบโต สารต้านอนุมูลอิสระ และความสามารถในการยับยั้งอนุมูลอิสระในไมโครกรีนผักขี้หูด (rat-tailed radish, Raphanus sativus var. caudatus Alef) ผักชีลาว (dill, Anethum graveolens L.) และแมงลัก (hairy basil, Ocimum africanum Lour.) โดยการพ่น ZnSO4 ความเข้มข้น 0, 0.5, 1.0, 1.5, 2.0 และ 2.5 µM เมื่ออายุ 2 วันหลังเพาะ หลังจากนั้นพ่นทุกวันจนถึงอายุ 11 วันหลังเพาะ และเก็บเกี่ยวเมื่ออายุ 12 วันหลังเพาะ พบว่าไมโครกรีนผักพื้นเมืองทุกชนิดมีน้ำหนักสดและน้ำหนักแห้งลดลงเมื่อความเข้มข้นของ ZnSO4 สูงขึ้น อย่างไรก็ตาม เมื่อพ่น ZnSO4 ความเข้มข้น 2.5 µM ส่งผลให้ไมโครกรีนทุกชนิดมีปริมาณสารประกอบฟีนอลิคทั้งหมดและฟลาโวนอยด์ทั้งหมดสูงที่สุด การพ่น ZnSO4 ความเข้มข้น 1.5 µM ส่งผลให้ไมโครกรีนผักขี้หูดและผักชีลาวมีความสามารถในการยับยั้งอนุมูลอิสระด้วยวิธี DPPH และ ABTS สูงสุด ขณะที่ไมโครกรีนแมงลักมีความสามารถในการยับยั้งอนุมูลอิสระทั้ง 2 วิธี สูงที่สุด เมื่อพ่นด้วย ZnSO4 ความเข้มข้น 1.0 µM นอกจากนี้ปริมาณไลโคปีนและเบตา-แคโรทีนในไมโครกรีนมีการตอบสนองต่อ ZnSO4 ต่างกัน ดังนั้นการใช้ปุ๋ยซัลเฟอร์ในรูป ZnSO4 เป็นอีกแนวทางหนึ่งในการเพิ่มปริมาณสารต้านอนุมูลอิสระ แคโรทีนอยด์ และความสามารถในการยับยั้งอนุมูลอิสระของไมโครกรีนผักพื้นเมืองทั้ง 3 ชนิดนี้
Article Details
ประเภทบทความ
Biological Sciences
เอกสารอ้างอิง
[1] Lertrat, K., Ngarmsak, M. and Sungsri-in, A., 2009, Thai food from local fruits and vegetables: The route to the sustainable well-being of Thai society, Khon Kaen Agr. J. 37: 1-20. (in Thai)
[2] Muchjajib, U., 2014, A Study on Commercial
Greenhouse Production of Local Plant Microgreens, Research Report, Raja mangala University of Technology Suvarnabhumi, Phra Nakhon Si Ayutthaya, 121 p. (in Thai)
[3] Xiao, Z., Lester, G.E., Luo, Y. and Wang, Q., 2012, Assessment of vitamin and carotenoid concentrations of emerging food products: Edible microgreens, J. Agric. Food Chem. 60: 7644-7651.
[4] Bunpatum, G. and Kethaisong, D., 2017, Evaluation of yield and bioactive compounds of thirteen vegetable microgreens, Khon Kaen Agr. J. 45: 368-373. (in Thai)
[5] Lester, G.E., Hallman, G.J. and Perez, J.A., 2010, r-Irradiation dose: Effects on baby-leaf spinach ascorbic acid, carotenoids, folate, -tocopherol, and phylloquinone concentrations, J. Agric. Food Chem. 58: 4901-4906.
[6] Oh, M.M., Carey, E.E. and Rajashekar, C.B., 2010, Regulated water deficits improve phytochemical concentration in lettuce, J. Am. Soc. Hort. Sci. 135: 223-229.
[7] Rice-Evans, C.A. and Miller, N.J., 1996, Antioxidant activities of flavonoids as bioactive compounds of foods, Biochem. Soc. T. 24: 790-795.
[8] Beanas, N., Garcia-Viguera, C. and Moreno, D.A., 2014, Elicitation: a tool for enhancing the bioactive composition of foods, Molecules 19: 13541-13563.
[9] Vallejo, F., Tomás-Barberán, F.A. and García-Viguera, C., 2003, Effect of climatic and sulphur fertilisation conditions, on phenolic compounds and vitamin C, in the inflorescences of eight broccoli cultivars, Eur. Food Res. Technol. 216: 395-401.
[10] Zhou, C., Zhu, Y. and Luo, Y., 2013, Effects of sulfur fertilization on the accumulation of health-promoting phytochemicals in radish sprouts, J. Agri. Food Chem. 61: 7752-7559.
[11] Smatanova, M., Richer, R. and Hlusek, J., 2004, Spinach and pepper response to nitrogen and sulphur fertilization, Plant Soil Environ. 50: 303-308.
[12] Schonhof, I., Blankenburg, D., Müller, S. and Krumbein, A., 2007, Sulfur and nitrogen supply influence growth, product appearance, and glucosinolate concentra tion of broccoli, J. Plant Nutr. Soil Sci. 170: 65-72.
[13] Perez-Balibrea, S., Moreno, D.A. and Garcia-Viguera, C., 2010, Glucosinolates in broccoli sprouts (Brassica oleracea var. italica) as conditioned by sulphate supply during germination, J. Food Sci. 5: C673-677.
[14] Li, J., Zhu, Z. and Gerendas, J., 2008, Effects of nitrogen and sulfur on total phenolics and antioxidant activity in two genotypes of leaf mustard, J. Plant Nutr. 31: 1642-1655.
[15] Kestwal, R.M., Lin, J.C., Bagal-Keswal, D.
and Chaing, B.H., 2011, Glucosinolates fortification of cruciferous sprouts by sulphur supplementation during cultiva tion to enhance anti-cancer activity, Food Chem. 126: 1164-1171.
[16] Losák, T., Hlušek, J., Kráěmar, S. and Varga, L., 2008, The effect of nitrogen and sulphur fertilization on yield and quality of kohlrabi (Brassica oleracea L.), R. Bras. Ci. Solo. 32: 697-703.
[17] Nualkaew, N., 2013, Study of biological activities of the extract from Thai local vegetables, Research Report, Thailand Research Fund, 247 p. (in Thai)
[18] Oonsivilai, R., Oonmetta-aree, J. and Singthong J., 2011, Bioactivity and Functional properties of Yanang, Krueo Manoy and Rang Chuet extracts, Research Report, Suranaree University of Technology, Nakhon Ratchasima, 44 p. (in Thai)
[19] Kubola, J., Siriamornpun, S. and Meeso, N., 2011, Phytochemicals, vitamin C and sugar content of Thai wild fruits, J. Agric. Food Chem. 126: 972–981.
[20] Nagata, M. and Yamashita, I., 1992, Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit, Nippon Shokuhin Kogyo Gakkaish 39: 925-928.
[21] Harakotr, B., Suriharn, B., Tangwongchai, R., Scott, M.P. and Lertrat, K., 2014, Anthocyanins and antioxidant activity in coloured waxy corn at different matura tion stages, J. Funct. Foods. 9: 109-118.
[22] Yang, R., Guo, L., Zhou, Y., Shen, C. and Gu, Z., 2015, Calcium mitigates the stress caused by ZnSO4 as a sulphur fertilizer and enhances the sulforaphane formation of broccoli sprouts, RSC Adv. 5: 12563-12570.
[23] De Pascale, S., Maggio, A., Pernice, R., Fagliano, V. and Barbieri, G., 2007, Sulphur fertilization may improve the nutritional value of Brassica rapa L. subsp. sylvestris, Eur. J. Agron. 26: 418-424.
[24] Kopsell, D.A., Barickman, T.C., Sams, C.E. and Mcelroy, J.C., 2007, Influence of nitrogen and sulfur on biomass produc tion and carotenoid and glucosinolate concentrations in watercress (Nasturtium officinale R. Br.), J. Agric. Food Chem. 55: 10628-10634.
[25] Tomas-Barberan, F.A. and Espin, J.C., 2001, Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables, J. Sci. Food Agric. 81: 853-876.
[26] Li, J., Zhu, Z.J. and Guo, S.R. Effects of nitrogen and sulfur application on antioxidant substances in leaf mustard, International Symposium on Vegetable Safety and Human Health, Beijing, China, February 28, 2010.
[27] Reif, C., Arrigoni, E., Scharer, H., Nystrom, L.
and Hurrell, R.F., 2013, Carotenoid database of commonly eaten Swiss vegetables and their estimated contribu tion to carotenoid intake. J. Food Com. Anal. 29: 64-72.
[28] Reif, C., Arrigoni, E., Neuweiler, R. Baumgartner, D., Nystrom, L. and Hurrell, R.F., 2012, Effect of sulfur and nitrogen fertilization on the content of nutritionally relevant carotenoids in spinach (Spinacia oleracea). J. Agri. Food Chem. 60: 5819-5824.
[29] Barros, L., Ferreira, M.J., Queiros, B., Ferreira, I.C.F.R. and Baptista, P., 2007, Total phenols, ascorbic acid, -carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chem. 103: 413-419.
[30] Murakami, M., Yamaguchi, T., Takamura, H. and Matoba, T., 2003, Effects of ascorbic acid and tocopherol on antioxidant activity of polyphenolic compounds. Food Chem. Toxicol. 68: 1622–1625.
[31] Najjaa, H., Zerria, K., Fattouch, S., Ammar, E. and Neffati, M., 2011, Antioxidant and antimicrobial activities of Allium roseum L. “Lazoul”, a wild edible endemic species in North Africa. Int. J. Food Prop. 14: 371-380.
[2] Muchjajib, U., 2014, A Study on Commercial
Greenhouse Production of Local Plant Microgreens, Research Report, Raja mangala University of Technology Suvarnabhumi, Phra Nakhon Si Ayutthaya, 121 p. (in Thai)
[3] Xiao, Z., Lester, G.E., Luo, Y. and Wang, Q., 2012, Assessment of vitamin and carotenoid concentrations of emerging food products: Edible microgreens, J. Agric. Food Chem. 60: 7644-7651.
[4] Bunpatum, G. and Kethaisong, D., 2017, Evaluation of yield and bioactive compounds of thirteen vegetable microgreens, Khon Kaen Agr. J. 45: 368-373. (in Thai)
[5] Lester, G.E., Hallman, G.J. and Perez, J.A., 2010, r-Irradiation dose: Effects on baby-leaf spinach ascorbic acid, carotenoids, folate, -tocopherol, and phylloquinone concentrations, J. Agric. Food Chem. 58: 4901-4906.
[6] Oh, M.M., Carey, E.E. and Rajashekar, C.B., 2010, Regulated water deficits improve phytochemical concentration in lettuce, J. Am. Soc. Hort. Sci. 135: 223-229.
[7] Rice-Evans, C.A. and Miller, N.J., 1996, Antioxidant activities of flavonoids as bioactive compounds of foods, Biochem. Soc. T. 24: 790-795.
[8] Beanas, N., Garcia-Viguera, C. and Moreno, D.A., 2014, Elicitation: a tool for enhancing the bioactive composition of foods, Molecules 19: 13541-13563.
[9] Vallejo, F., Tomás-Barberán, F.A. and García-Viguera, C., 2003, Effect of climatic and sulphur fertilisation conditions, on phenolic compounds and vitamin C, in the inflorescences of eight broccoli cultivars, Eur. Food Res. Technol. 216: 395-401.
[10] Zhou, C., Zhu, Y. and Luo, Y., 2013, Effects of sulfur fertilization on the accumulation of health-promoting phytochemicals in radish sprouts, J. Agri. Food Chem. 61: 7752-7559.
[11] Smatanova, M., Richer, R. and Hlusek, J., 2004, Spinach and pepper response to nitrogen and sulphur fertilization, Plant Soil Environ. 50: 303-308.
[12] Schonhof, I., Blankenburg, D., Müller, S. and Krumbein, A., 2007, Sulfur and nitrogen supply influence growth, product appearance, and glucosinolate concentra tion of broccoli, J. Plant Nutr. Soil Sci. 170: 65-72.
[13] Perez-Balibrea, S., Moreno, D.A. and Garcia-Viguera, C., 2010, Glucosinolates in broccoli sprouts (Brassica oleracea var. italica) as conditioned by sulphate supply during germination, J. Food Sci. 5: C673-677.
[14] Li, J., Zhu, Z. and Gerendas, J., 2008, Effects of nitrogen and sulfur on total phenolics and antioxidant activity in two genotypes of leaf mustard, J. Plant Nutr. 31: 1642-1655.
[15] Kestwal, R.M., Lin, J.C., Bagal-Keswal, D.
and Chaing, B.H., 2011, Glucosinolates fortification of cruciferous sprouts by sulphur supplementation during cultiva tion to enhance anti-cancer activity, Food Chem. 126: 1164-1171.
[16] Losák, T., Hlušek, J., Kráěmar, S. and Varga, L., 2008, The effect of nitrogen and sulphur fertilization on yield and quality of kohlrabi (Brassica oleracea L.), R. Bras. Ci. Solo. 32: 697-703.
[17] Nualkaew, N., 2013, Study of biological activities of the extract from Thai local vegetables, Research Report, Thailand Research Fund, 247 p. (in Thai)
[18] Oonsivilai, R., Oonmetta-aree, J. and Singthong J., 2011, Bioactivity and Functional properties of Yanang, Krueo Manoy and Rang Chuet extracts, Research Report, Suranaree University of Technology, Nakhon Ratchasima, 44 p. (in Thai)
[19] Kubola, J., Siriamornpun, S. and Meeso, N., 2011, Phytochemicals, vitamin C and sugar content of Thai wild fruits, J. Agric. Food Chem. 126: 972–981.
[20] Nagata, M. and Yamashita, I., 1992, Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit, Nippon Shokuhin Kogyo Gakkaish 39: 925-928.
[21] Harakotr, B., Suriharn, B., Tangwongchai, R., Scott, M.P. and Lertrat, K., 2014, Anthocyanins and antioxidant activity in coloured waxy corn at different matura tion stages, J. Funct. Foods. 9: 109-118.
[22] Yang, R., Guo, L., Zhou, Y., Shen, C. and Gu, Z., 2015, Calcium mitigates the stress caused by ZnSO4 as a sulphur fertilizer and enhances the sulforaphane formation of broccoli sprouts, RSC Adv. 5: 12563-12570.
[23] De Pascale, S., Maggio, A., Pernice, R., Fagliano, V. and Barbieri, G., 2007, Sulphur fertilization may improve the nutritional value of Brassica rapa L. subsp. sylvestris, Eur. J. Agron. 26: 418-424.
[24] Kopsell, D.A., Barickman, T.C., Sams, C.E. and Mcelroy, J.C., 2007, Influence of nitrogen and sulfur on biomass produc tion and carotenoid and glucosinolate concentrations in watercress (Nasturtium officinale R. Br.), J. Agric. Food Chem. 55: 10628-10634.
[25] Tomas-Barberan, F.A. and Espin, J.C., 2001, Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables, J. Sci. Food Agric. 81: 853-876.
[26] Li, J., Zhu, Z.J. and Guo, S.R. Effects of nitrogen and sulfur application on antioxidant substances in leaf mustard, International Symposium on Vegetable Safety and Human Health, Beijing, China, February 28, 2010.
[27] Reif, C., Arrigoni, E., Scharer, H., Nystrom, L.
and Hurrell, R.F., 2013, Carotenoid database of commonly eaten Swiss vegetables and their estimated contribu tion to carotenoid intake. J. Food Com. Anal. 29: 64-72.
[28] Reif, C., Arrigoni, E., Neuweiler, R. Baumgartner, D., Nystrom, L. and Hurrell, R.F., 2012, Effect of sulfur and nitrogen fertilization on the content of nutritionally relevant carotenoids in spinach (Spinacia oleracea). J. Agri. Food Chem. 60: 5819-5824.
[29] Barros, L., Ferreira, M.J., Queiros, B., Ferreira, I.C.F.R. and Baptista, P., 2007, Total phenols, ascorbic acid, -carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food Chem. 103: 413-419.
[30] Murakami, M., Yamaguchi, T., Takamura, H. and Matoba, T., 2003, Effects of ascorbic acid and tocopherol on antioxidant activity of polyphenolic compounds. Food Chem. Toxicol. 68: 1622–1625.
[31] Najjaa, H., Zerria, K., Fattouch, S., Ammar, E. and Neffati, M., 2011, Antioxidant and antimicrobial activities of Allium roseum L. “Lazoul”, a wild edible endemic species in North Africa. Int. J. Food Prop. 14: 371-380.
