Antibacterial, Antioxidant and Anticancer Activities of the Caesalpinia sappan L. Extract
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Abstract
The purposes of this research were to investigate antibacterial activity, antioxidant activity and anti-cancer activities against human cell lines, KB oral cavity cancer and NCI-H187 small lung cancer of the methanolic extract of Caesalpinia sappan Linn. heartwood. The antibacterial activity was examined by using agar well diffusion method. The antioxidant activity was determined using DPPH radical scavenging assay. The anti-cancer activities against KB oral cavity cancer and NCI-H187 small lung cancer were analyzed by the Bio-resources Technology Unit (BTU), National Center for Genetic Engineering and Biotechnology (BIOTEC). The research results were that the methanolic extract of C. sappan heartwood exhibited strong antibacterial activities against Staphylococcus aureus (ATCC25923), Salmonella Typhimurium (ATCC13311) and Vibrio cholera (non 01, non 0139, DMST2873) with MIC values of 0.313, 0.500 and 0.625 mg/mL, respectively. For analyzing the antioxidant activity, it was found that the methanolic extract was about 13.15 times higher antioxidant activity (IC50 = 35.26±2.08 µg/mL) than that of BHT (butylated hydroxytoluene) standard (IC50 = 463.70±34.20 µg/mL). Moreover, the methanolic extract at a concentration of 50 µg/mL exhibited anti-cancer activities against human cell lines, KB oral cavity cancer and NCI-H187 small lung cancer with inhibition activities of 78.08 and 80.75 %, respectively.
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