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The aim of this study was to evaluate whether monoglyceride (MG) extracted from virgin coconut oil (VCO) has a fungicidal effect on both fluconazole-susceptible and fluconazole-resistant strains of C. albicans, comparing with VCO. Both strains of C. albicans were evaluated with 18% and 36% (v/v) MG using broth micro-dilution technique with concentrations ranging from 10-6-101 CFU/mL. The experiment was performed in 96-well tissue-culture plates. After 24-hr incubation at 35 °C, each solution was cultured on Sabouraud dextrose agar for another 24-hr to evaluate the fungicidal effects. The 36 % MG was selected as the most effective MG concentration and then used to compare the fungicidal effect with a 3-week prepared 36 % MG, 36 % VCO, 8 µg/ml and 0.25 µg/ml fluconazole. The evaluation was tested with the broth micro-dilution. The present study showed that 36 % MG extracted has a fungicidal ability as similar effect as fluconazole to kill both susceptible and fluconazole-resistant strains of C. albicans at 103 CFU/mL, while 18 % MG demonstrated this ability at 102 CFU/mL. Comparing 36 % MG with other groups, the 3-week prepared 36% MG demonstrated a less fungicidal ability at 102 CFU/mL. Interestingly, the fungicidal effect was not observed in the 36 % VCO. Thirty-six percent of MG extracted from VCO demonstrated a fungicidal ability as similar effect as fluconazole against both susceptible and fluconazole-resistant strains of C. albicans at 103 CFU/mL, which was higher than 18 % MG and 3-week prepared 36 % MG. Of note, there was no fungicidal effect of 36 % VCO.
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