การสร้างภาพสามมิติของการเจริญบริเวณอุ้งเชิงกรานในตัวอ่อนไก่อายุ 9 วัน

Abstract

Development of organs in organisms has been studied for long time. They have been studied by both serial sections and whole-mount under microscopic investigation to demonstrate the developmental processes in either micrographs or drawings. Although the developmental processes are well defined, they remain ignoring the dimension. Nowadays, the technology called 3D reconstruction increases the capability of embryological studies including the third dimension (z-axis or thickness) and volumetric data to visualize the developmental mechanism in natural environment. This research aims to apply the 3D reconstruction and morphometry to illustrate the normal development of pelvic region in 9-day chick embryo. The serial sections were imported and analyzed histologically and volumetrically in Amira 3D software. The 3D model was further remodelled in Cinema 4D program to decrease sectioning noise which disrupt the perspective. The 3D model from the reconstruction showed that it can rotate to visualize the topography of the pelvic structures and compare the volume of organs in pelvic region. The main results found that a created 3D image reconstruction can visualize the pelvic region structure close to the real embryo structure. Moreover, the differentiation of the pelvic region structure at the tissue level can be illustrated by a created 3D image reconstruction. Thus, this created 3D image reconstruction is able be used as a tool for demonstrating the morphological changes in embryonic development.