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Sugarcane white leaf is a major disease of sugarcane caused by phytoplasma and causing severe damage to sugarcane production in almost all regions of the country. The fifty-seven sugarcane fields at Aranyaprathet district, Sakaeo province, were surveyed. The results showed that sugarcane in thirteen areas located in Han Sai, Nong Sang, Khlong Nam Sai, Thap Phrik, Phan Suek, Khlong Thap Chan, and Muang Phai sub-districts, was found white leaf disease. The white leaf samples collected from the sugarcane field were analyzed for phytoplasma infection by PCR technique. All infected samples were positive with PCR detection, and the nucleotide sequences of 16S rRNA gene showed 98-99 % similarity with sugarcane white leaf disease. Therefore, the data were applied on geographic information system as an effective tool to present in spatial data and annotate a thematic map of sugarcane white leaf disease in the sugarcane field in the year 2019 at Aranyaprathet district, Sakaeo province. The present study results were basic information to be used as a decision support system for sugarcane planting, white leaf disease infection preventing, and not bringing infected sugarcane to grow in the field.
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