Sugarcane White Leaf Disease Survey in Aranyaprathet District, Sakaeo Province

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อารมย์ จันทะสอน
ศศิธร โคสุวรรณ
ธนประสงค์ อยู่พิศิษฐ์ไตรวัติ
สิทธิศักดิ์ แสไพศาล

Abstract

Sugarcane white leaf is a major disease of sugarcane caused by phytoplasma and causing severe damage to sugarcane production in almost all regions of the country. The fifty-seven sugarcane fields at Aranyaprathet district, Sakaeo province, were surveyed. The results showed that sugarcane in thirteen areas located in Han Sai, Nong Sang, Khlong Nam Sai, Thap Phrik, Phan Suek, Khlong Thap Chan, and Muang Phai sub-districts, was found white leaf disease. The white leaf samples collected from the sugarcane field were analyzed for phytoplasma infection by PCR technique. All infected samples were positive with PCR detection, and the nucleotide sequences of 16S rRNA gene showed 98-99 % similarity with sugarcane white leaf disease. Therefore, the data were applied on geographic information system as an effective tool to present in spatial data and annotate a thematic map of sugarcane white leaf disease in the sugarcane field in the year 2019 at Aranyaprathet district, Sakaeo province. The present study results were basic information to be used as a decision support system for sugarcane planting, white leaf disease infection preventing, and not bringing infected sugarcane to grow in the field.

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วิทยาศาสตร์ชีวภาพ
Author Biographies

อารมย์ จันทะสอน

วิทยาลัยโพธิวิชชาลัย มหาวิทยาลัยศรีนครินทรวิโรฒ อำเภอองครักษ์ จังหวัดนครนายก 26120

ศศิธร โคสุวรรณ

วิทยาลัยโพธิวิชชาลัย มหาวิทยาลัยศรีนครินทรวิโรฒ อำเภอองครักษ์ จังหวัดนครนายก 26120

ธนประสงค์ อยู่พิศิษฐ์ไตรวัติ

วิทยาลัยโพธิวิชชาลัย มหาวิทยาลัยศรีนครินทรวิโรฒ อำเภอองครักษ์ จังหวัดนครนายก 26120

สิทธิศักดิ์ แสไพศาล

ศูนย์วิจัยและพัฒนาการเกษตรระนอง ตำบลบางใหญ่ อำเภอกระบุรี จังหวัดระนอง 85110

References

Office of Cane and Sugar Board, Annual Report, Available Source: http://www.ocsb.go.th/upload/journal/fileupload/923-9040.pdf, January 20, 2020. (in Thai)

Taweekull, N., Sansayawichai, T. and Kirasak, K., 2012, Sugar cane white leaf disease management through phytoplasma-free seed cane, Khon Kaen Agric. J. 40(Suppl. 3): 241-248. (in Thai)

Hanboonsong, Y., Ritthison, W., Choosai, C. and Sirithorn, P., 2006, Transmission of sugarcane white leaf phytoplasma by Yamatotettix flavovittatus, a new leaf hopper vector, J. Econ. Entomol. 99: 1531-1537.

Jantasorn, A., Kosuwan, S., Somrit, M., Saepaisal, S. and Oiuphisittraiwat, T., 2017, Application of Geographic information system for determining the distribution of papaya ringspot virus in commercial papaya plantation area at Sakaeo province, SWU Sci. J. 33(1): 73-85. (in Thai)

Bunlue, B., 2010, Applications of Geo-informatics in monitoring forest land use changes in Khao Ang Rue Nai Wildlife Sanctuary, Master’s Thesis, Kasetsart University, Bangkok, 100 p. (in Thai)

Doydee, P. and Waesorn, S. 2015, Applica tion of geographic information system for selected commercial crop zonation mapping in Nong Han Luang, Sakon Nakhon province, Thailand, pp. 1552-1560, The 53th Kasetsart University Annual Conference, Bangkok. (in Thai)

Michael, L. and Amy, C., 2013. ArcGIS for Desktop. 1st Ed., Esri Press, New York.

Thayaping, P. and Suttiprapan, P., 2015, Geo-information technology application for studying species diversity and distribution of arabica coffee insect pests in Chiangm Mai and Chiang Rai provinces, J. Agric. 3(2): 203-213. (in Thai)

Hanboonsong, Y., Ritthison, W. and Choosai, C., 2005, Molecular detection and transmission of sugarcane white leaf phytoplasma on leafhoppers, KKU Res. J. 10(1): 13-21. (in Thai)

Gundersen, D. and Lee, I.M., 1996, Ultrasensitive detection of phytoplasma by nested PCR assays using two universal primer pairs, Phytopath. Medit. 35: 114-151.

Khan, J.A., Srivastava, P. and Singh, S.K., 2004, Efficacy of nested PCR for the detection of phytoplasma causing spike disease of sandal, Curr. Sci. 86: 1530-1533.

Srivastava, S., Singh, V., Gupta, P.S., Sinha, O.K. and Baitha, A., 2006, Nested PCR assay for detection of sugarcane grassy shoot phytoplasma in the leafhopper vector Deltocephallus vulgaris, a first report. Plant Pathol. 55: 25-28.

Parmessure, Y., Aljanabi, S., Saumtally, S. and Dookum-Saumtally, A., 2002, Sugarcane yellow leaf virus and sugarcane yellow phytoplasma: Elimination by tissue culture, Plant Pathol. 51: 561-566.

Chen, C.T., 1973, Insect transmission sugarcane white leaf disease by single leafhopper Matsumuratettix hiroglyphicus (Matsumura), Taiwan Sugar Rec. Inst. 60: 25-33.

Chen, C.T., 1978, Vector pathogen relationships of sugarcane white leaf disease, Taiwan Sugar J. 25: 50-54.

Hanboonsong, Y., Choosai, C., Panyim, S. and Damak, S., 2002, Transovarial trans mission of sugarcane white leaf phyto plasma in the insect vector Matsumura tettix hiroglyphicus (Matsumura), Insect Mol. Biol. 11: 97-103.

Rattanaboontha, C., Hanboonsong, Y. and Khibsuwan, W., 2012, Logistic regression model for forecasting sugarcane white leaf disease, Khon Kaen Agric. J. 40(Suppl.): 443-448. (in Thai)

Huadprasit, J., Wangkeeree, J. and Hanboonsong, Y., 2017, Efficiency of Metarhizium and Beauveria to control the leafhopper vector, Matsumuratettix hiroglyphicus, of sugarcane white leaf, Thai Sci. Technol. J. 25(3): 467-478. (in Thai)