Effects of Methyl Jasmonate and Salicylic Acid on Antioxidant Contents in Callus Cultures of Holy Basil Purple Type

Main Article Content

ณัฐนรี ไพสิฐสกุลนาท
เยาวพา จิระเกียรติกุล
ภาณุมาศ ฤทธิไชย

Abstract

In vitro callus culture with elicitation is one of the most popular methods to enhance antioxidant contents. This study aimed to investigate the effects of methyl jasmonate (MeJA) and salicylic acid (SA) at various concentrations on total phenolic and flavonoid contents, and antioxidant activity in callus cultures of holy basil purple type (Ocimum santum). Callus was cultured on MS (Murashige and Skoog) medium supplemented with 2.26 µM 2,4-D (2,4-dichlorophenoxyacetic acid) combined with MeJA and SA at the concentrations of 0-150 µM for two weeks. The experiment was arranged in a completely randomized design with seven treatments and three replications. Total phenolic contents of 144.76±8.19 and 134.56±13.10 mg GAE/g dry extract or 2.92 and 2.71 times of the control (49.65±1.88 mg GAE/g dry extract) were obtained from 100 and 150 µM MeJA calli. Flavonoid contents of 215.77±23.50 and 221.72±9.75 mg CE/g dry extract were also gained from 100 and 150 µM MeJA calli or 2.71 and 2.78 times of the control (79.67±3.88 mg CE/g dry extract). In addition, DPPH radical scavenging activities of 100 and 150 µM MeJA calli (EC50 = 9.06±0.45 and 9.33±0.65 µg/mL) were greater than that of the control (EC50 = 20.72±2.06 µg/mL). Therefore, 100 and 150 µM MeJA could enhance antioxidant contents of holy basil purple type callus after cultured for two weeks.

Downloads

Download data is not yet available.

Article Details

Section
วิทยาศาสตร์ชีวภาพ
Author Biographies

ณัฐนรี ไพสิฐสกุลนาท

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

เยาวพา จิระเกียรติกุล

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

ภาณุมาศ ฤทธิไชย

สาขาวิชาเทคโนโลยีการเกษตร คณะวิทยาศาสตร์และเทคโนโลยี มหาวิทยาลัยธรรมศาสตร์ ศูนย์รังสิต ตำบลคลองหนึ่ง อำเภอคลองหลวง จังหวัดปทุมธานี 12120

References

Singh, D. and Chaudhuri, P.K., 2018, A review on phytochemical and pharmacological properties of holy basil (Ocimum sanctum L.), Ind. Crops Prod. 118: 367-382.

Singh, S., Majumdar, D.K. and Rehan, H.M.S., 1996, Evaluation of anti-inflammatory potential of fixed oil of Ocimum sanctum (holy basil) and its possible mechanism of action, J. Ethnopharmacol. 54: 19-26.

Siva, M., Shanmugam, K.R., Shanmugam, B., Venkata, S.G., Ravi, S., Sathyavelu, R.K. and Mallikarjuna, K., 2016, Ocimum sanctum: A review on the pharmaco logical properties, Int. J. Basic Clin. Pharmacol. 5: 558-565.

Putalun, W., 2008, Medicinal Plant Tissue Culture: Study Guidelines for Production of Secondary Metabolites, Department of Pharmacognosy and Toxicology, faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 120 p. (in Thai)

Lardee, N., 2018, Effect of Different Culture Periods on Secondary Metabolite Contents of Ocimum sanctum L. (Holy Basil Purple Type) Callus, Special Problem, Thammasat University, Pathum Thani, 29 p. (in Thai)

Bunrathep, S., 2006, Production of secondary metabolites by plant tissue cultures and biological technology, Thai J. Health Res. 20: 185-195. (in Thai)

Zhao, J., Davis, L.C. and Verpoorte, R., 2005, Elicitor signal transduction leading to production of plant secondary metabolites, Biotechnol. Adv. 23: 283-333.

Martínez, V.M.V., Estrada-Soto, S.E., Arellano-García, J.J., Rivera-Leyva, J.C., Castillo-España, P., Flores, A.F., Cardoso-Taketa, A.T. and Perea-Arango, I., 2017, Methyl jasmonate and salicylic acid enhanced the production of ursolic and oleanolic acid in callus cultures of Lepechinia Caulescens, Phcog. Mag. 13: 886-889.

Pandey, H., Pandey, P., Singh, S., Gupta, R. and Banerjee, S., 2015, Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation, Protoplasma. 252: 647-655.

Udomsuk, L., Jarukamjorn, K., Tanaka, H. and Putalun, W., 2011, Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation, Biotechnol. Lett. 33: 369-374.

Abeda, H.Z., Kouassi, M.K., Yapo, K.D., Koffi, E., Sie, R.S., Kone, M. and Kouakou, H.T., 2014, Production and enhancement of anthocyanin in callus line of roselle (Hibiscus sabdariffa L.), Int. J. Rec. Biotech. 2: 45-56.

Folin, O. and Ciocalteu, V., 1927, On tyrosine and tryptophane determinations in proteins, J. Biol. Chem. 73: 627-650.

Zhu, H., Wang, Y., Liu, Y., Xia, Y. and Tang, T., 2010, Analysis of flavonoids in Portulaca oleracea L. by UV-Vis spectrophotometry with comparative study on different extraction technologies, Food Anal. Methods 3: 90-97.

Yamasaki, K., Hashimoto, A., Kokusenya, Y., Miyamoto, T. and Sato, T., 1994, Electrochemical method for estimating the antioxidative effects of methanol extracts of crude drugs, Chem. Pharm. Bull. 42: 1663-1665.

Naik, P.M. and Al-Khayri, J.M., 2016, Abiotic and Biotic Elicitors-Role in Secondary Metabolites Production Through In vitro Culture of Medicinal Plants, pp. 247-277, In Shanker, A. and Shanker, C. (Eds.), Abiotic and Biotic Stress in Plants, Intech, London.

Bari, R. and Jones, J.D.G., 2009, Role of plant hormones in plant defence responses, Plant Mol. Biol. 69: 473-488.

Namedo, A.G., 2007, Plant cell elicitation for production of secondary metabolites: A review, Pharmacogn. Rev. 1: 69-79.

Sudha, G. and Ravishankar, G.A., 2003, Elicitation of anthocyanin production in callus cultures of Daucus carota and the involvement of methyl jasmonate and salicylic acid, Acta Physiol. Plant. 25: 249-256.

Hakkim, F.L., Kalyani, S., Essa, M., Girija, S. and Song, H., 2011, Production of rosmarinic in Ocimum sanctum cell cultures by the influence of sucrose, phenylalanine, yeast extract, and methyl jasmonate, Int. J. Biol. Med. Res. 2: 1070-1074.

Cheong, J.J. and Choi, Y.D., 2003, Methyl jasmonate as a vital substance in plants, Trends Genet. 19: 409-413.

Wang, J., Qian, J., Yao, L. and Lu, Y., 2015, Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum, Bioresour. Bioprocess 2: 1-9.

Jalalpour, Z., Shabani, L., Afghani, L., Sharifi-Tehrani, M. and Amini, S.A., 2014, Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew, Turk. J. Biol. 38: 76-82.

Giri, L., Dhyani, P., Rawat, S., Bhatt, I.D., Nandi, S.K., Rawal, R.S. and Pande, V., 2012, In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: Arare Himalay an medicinal orchid, Ind. Crops Prod. 39: 1-6.

Lee, E.J., Park, S.Y. and Paek, K.Y., 2015, Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors, Plant Cell Tiss. Org. Cult. 120: 1-10.

Minatel, I.O., Borges, C.V., Ferreira, M.I., Gomez, H.A.G., Chen, C.Y.O. and Lima, G.P.P., 2017, Phenolic Compounds: Functional Properties, Impact of Processing and Bioavailability, pp. 1-24, In Soto-Hernández, M. (Ed.), Phenolic Compounds Biological Activity, IntechOpen, Ltd., London.

Korkina, L.G. and Afanas'ev, I.B., 1996, Antioxidant and chelating properties of flavonoids, Adv. Pharmacol. 38: 151-163.

Manivannan, A., Soundararajan, P., Park, Y.G. and Jeong, B.R., 2016, Chemical elicitor-induced modulation of antioxidant metabolism and enhancement of secondary metabolite accumulation in cell suspension cultures of Scrophularia kakudensis Franch, Int. J. Mol. Sci. 17: 1-13.

Shilpha, J., Satish, L., Kavikkuil, M., Largia, M.J.V. and Ramesh, M., 2015, Methyl jasmonate elicits the solasodine production and anti-oxidant activity in hairy root cultures of Solanum trilobatum L. Ind. Crops Prod. 71: 54-64.

Danaee, M., Farzinebrahimi, R., Kadir, M.A., Sinniah, U.R., Mohamad, R. and Taha, R.M., 2015, Effects of MeJA and SA elicitation on secondary metabolic activity, antioxidant content and callogenesis in Phyllanthus pulcher, Braz. J. Bot. 38: 265-272.

Yi, T.G., Park, Y., Park, J.E. and Park, N.I., 2019, Enhancement of phenolic compounds and antioxidative activities by the combination of culture medium and methyl jasmonate elicitation in hairy root cultures of Lactuca indica L, Nat. Prod. Commun. 14(7): 9 p.