Effects of Chitosan Films and Temperatures on Shelf Life of Kratom (Mitragyna speciosa (Korth.) Havil) Leaves

Main Article Content

Rawee Chiarawipa
Wethanee Phromchan
Adirek Rugkong

Abstract

Kratom is a traditional medicinal plant widely consumed as fresh green leaves. As kratom leaves degrade rapidly after harvesting, preservation is required to prolong the shelf life and quality of the leaves. In this study, the temperatures used to store kratom leaves were 15°C and 25°C (room temperature), and the five levels of chitosan concentration (0.0, 0.5, 1.0, 1.5, and 2.0%) were applied to the leaves before the storage. It was found that the storage of the leaves at 15°C with 0.5% chitosan concentration resulted in a lower weight loss percentage than the storage at 25°C. The storages of kratom leaves at 15, and 25°C showed a significant difference in leaf color (a*, b*, and L*). However, the different concentrations of chitosan applications caused no difference in leaf color change. Regarding the chlorophyll content, it was found that the storage at 15°C with varying concentrations of chitosan resulted in different values of leaf chlorophyll. The values ranged between 20.05 and 26.57 mg/cm2, lower than those of the 25°C storage, which were in the range of 19.44- 34.06 mg/cm2. The results showed that the shelf life period of kratom leaves was associated with low temperature and chitosan application. The storage of kratom leaves at 15°C with 0.5% chitosan extended the shelf life by approximately 15 days longer than the storage at 25 °C. Therefore, it indicates that the effective packaging system to minimize phytochemical changes during the storage period should be studied further.

Article Details

Section
Biological Sciences

References

Panyaphu, D., Namkeard, S., Inchai, W. and Yossathera, K., 2016, Kratom: Herbal medicine or narcotic drug, J. Thai. Trad. Alt. Med. 14: 242-256.

Akaphan, T., Teerachaisakul, M., Puttum, S., Sompimai, K. and Nootim, P., 2016, Traditional uses of kratom (Mitragyna speciosa Korth.) among folk healers in southern Thailand, J. Thai. Trad. Alt. Med.14: 274-285.

Kuriwan, O., 2017, Purification and determination of mitragynine from Mitragyna speciosa Korth leaves Pathumthani province, Master Thesis, Silpakorn University, Nakhon Pathom, (in Thai)

Muandao, K., 2018, Comparative extraction method of mitragynine for forensic science and their antibacterial properties of Mitragyna speciosa Korth., Master Thesis, Silpakorn University, Nakhon Pathom, (in Thai)

Purintrapiban, J., Keawpradub, N., Kansenalak, S., Chittrakarn, S., Janchawee, B. and Sawangjaroen, K. 2011. Study on glucose transport in muscle cells by extracts from Mitragyna speciosa (Korth.) and mitragynine, Nat. Prod. Res. 25: 1379-1387.

Todd, D.A., Kellogg, J.J., Wallace, E.D., Khin, M., Flores Bocanegra, L., Tanna, R.S., McIntosh, S., Raja, H.A., Graf, T.N., Hemby, S.E., Paine, M.F., Oberlies, N.H. and Cech, N.B., 2020, Chemical composition and biological effects of kratom (Mitragyna speciosa): In vitro studies with implications for efficacy and drug interactions, J. Nat. Sci. Res. DOI: 10.1038/s41598-020-76119-w

Ilmie, M.U., Mansor, S.M. and Abdullah, J.M., 2015, Behavioural and electrophysiological evidence of impaired learning and memory in male sprague dawley rats following subchronic exposure to standardised methanolic extract of Mitragyna speciosa Korth, Malays J. Med. Sci. 22:45-51.

Chanjula, P., Wungsintaweekul, J., Chiarawipa, R., Rugkong, Khonkhaeng, B., A., Suntara, C. and Cherdthong, A., 2021, Effect of feed supplement containing dried kratom leaves on apparent digestibility, rumen fermentation, serum antioxidants, hematology, and nitrogen balance in goats, Fermentation, 8(3): 131. DOI:10.3390/fermentation8030131.

Seidl, P.R. 1999. Prospects for Brazilian natural products. Anais da Academia Brasileira de Ciencias. 71: 239-247.

Department of Primary Industries and Reional Development, 2016, Storage of Fresh Fruit and Vegetables, Available Source: https://www.agric.wa.gov.au/fruit/storage-freshfruit-and-vegetables, July 8, 2021.

Romanazzi, G., Feliziani, E., Baños, S.B. and Sivakumar, D., 2015, Shelf life extension of fresh fruit and vegetables by chitosan treatment, Crit. Rev. Food. Sci. Nutr. DOI: 10.1080/10408398.2014.900474.

Devlieghere, F., Vermeulen, A., and Debevere, J., 2004, Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables, Food Microbiol. 21:703-714.

Fisk, C.L., Silver, A.M., Strik, B.C. and Zhao Y., 2008, Postharvest quality of hardy kiwifruit (Actinidia arguta “Ananasnaya”) associated with packaging and storage conditions, Postharvest Biol. Technol. 47:338-345.

Chootummatat, S., Kangkamanee, S., Dum-ampai, N., Suphatthra, L., Rugkong, A., Chanaweerawan, S., Surawut, A., Joodkong, A., Sankaew, N., Tunthai, P., Tirapat, P., Chareaunsang, U., Charigkapakorn, N. and Patchimkul, S., 2011, Testing of appropriate stage of fruit set by chitosan coating at pre-harvest to pro-long storage life of longkong (Lansium domesticum Corr.), Available Source: https://www.doa.go.th/hort/, July 7, 2021. (in Thai)

Kwanhong, P. and Ratanachinakorn, B., 2014, Effect of low concentration of chitosan on storage quality of rose apple cv. Thong Sam Sri, Khon Kaen Agr. J. 42:180-185. (in Thai)

Pengnet, N., Sang-On, B. and Chaiprasart, P., 2015, Effect of a chitosan-based coaing on physical and chemical change of fresh-cut mangoes (Mangifera indica L.) cv. Nam Dok Mai#4 stored at 5๐C, Agrcultural Sci. J. 46: 172-175. (in Thai)

Aiamla-or, S., Uthairatanakij, A., Jitareerat, P. and Pasanphan, W., 2015, Effect of nano-chitosan coating on physical and chemical changes of gamma ray lrradiated mango cv. ‘Nam Dok Mai No.4’, Agricultural Sci. J. 46: 109-112. (in Thai)

Luo, T., Li, S., Han, D., Guo, X., Shuai, L. and Wu, Z., 2018, The effect of desulfurization on the postharvest quality and sulfite metabolism in pulp of sulfitated “Feizixiao” Litchi (Litchi chinensis Sonn.) fruits, Food Sci. Nutr. 7: 1715-1726.

Hamathulin, A., Simla, S., Boontang, S. and Inchuen, S., 2012, Relationship between color value and anthocyanin content in purple waxy corn germplasm, Khon Kaen Agr. J. 4: 59-64.

Chiarawipa, R. and Sirikantayakul, C., 2015, Phenotypic plasticity in Robusta coffee trees in a mixed orchard system, Agricultural Sci. J. 46: 433-436. (in Thai)

Bunya-atichar, K., 2017, Effects of low temperatures and storage duration on shelf life of ready to cook melinjo (Gnetum gnemon L.) leaves, Songklanakarin J. Pl. Sci. 4: 55-59. (in Thai)

Lattanzio, V., Lattanzio, V.M.T. and Cardinali, A., 2006, Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects, Recent Adv. Phytochem. 2: 23-67.

Watada, A.E., Ko, N.P. and Minott, D.A., 1996, Factors affecting quality of freshcut horticultural products, Postharvest Biol. Technol. 9: 115-125.

Schmitz, F.R.W., Carvalho, L.F.D., Bertoli, S.L. and Souza, C.K.D., 2019, Effect of refrigerated storage conditions on leafy vegetables, MOJ Food Process Technol.7: 75-77.

Badawy, M.E.I. and Rabea, E.I., 2009, Potential of the biopolymer chitosan with different molecular weights to control postharvest gray mold of tomato fruit, Postharvest Biol. Technol. 51: 110-117.

Poubol, J. and Boonyaritthongchai, P., 2011, Chitosan coating on physiological and quality changes of asparagus spears, J. Agric. Sci. 42: 569-572. (in Thai)

Zhang, D. and Quantick, P.C., 2015, Antifungal effects of chitosan coating on fresh strawberries and raspberries during storage, J. Hortic. Sci. 73: 763-767.

Hernandez-Munoz, P., Almenar, E., Ocio, M.J. and Gavara, R., 2006, Effect of calcium dips and chitosan coatings on postharvest life of strawberries (Fragaria × ananassa), Postharvest Biol. Technol. 39:247-253.

Altieri, C., Scroco, C., Sinigaglia, M. and Nobile, M.A.D., 2005, Use of chitosan to prolong mozzarella cheese shelf life, Int. J. Dairy Sci. 88: 2683-2688.

Kittur, F.S., Saroja Habibunnisa, N.S. and Tharanathan, R.N., 2001, Polysaccharide-based composite coating formulations for shelf-life extension of fresh banana and mango, Eur. Food Res. Technol. 213: 306-311.

Baez-sanudo, M., Siller-Cepeda, J., Muy-Rangel, D. and Heredia, J.B., 2009, Extending the shelf-life of bananas with 1-methyl cyclopropene and a chitosan-basededible coating, J. Sci. Food Agric. 89: 2343–2349.

Lueangprasert, K. and Puttha, R., 2015, Effects of leaf-age stages on postharvest quality changes of leaf chicory during

storage, Agricultural Sci. J. 46: 137-140. (in Thai)