Domoic Acid in Stranded Green Turtle in Andaman Coast of Thailand

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Thitima Niyomsilpchai
Supanoi Subsinserm
Narumol Kornkanitnan
Piyarat Khumraksa
Rachawadee Chantra


Exposure to domoic acid was determined in 12 stranded green turtles on the Andaman coast, Thailand from March-November 2020. The results revealed that 92% (11/12) of the stranded green turtles were positive for domoic acid concentrations ranging between not detected and 16.24 μg/g. The highest average concentration was detected in the liver (2.61±4.46 μg/g), followed by the kidneys (2.49±5.20 μg/g), stomach contents (1.17±1.65 μg/g), feces (0.92±1.63 μg/g), and muscles (0.85±1.45 μg/g). In many countries, exposure to biotoxins, produced by phytoplankton can result in unusual strandings (dead and alive) of marine wildlife. However, in Thailand, this study is the first report of domoic acid in stranded sea turtles. More research is needed for a better understanding of the phytoplankton biotoxins in the marine food web and their impact on sea turtles, which are marine endangered species in Thailand.

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Biological Sciences


Marine and Coastal Resources Research and Development Institute, Department of Marine and Coastal Resources. Marine Endangered Species Stranding Response Manual. Available Sources: https://www.dmcr.go. th/detailLib/6245. 112 p., March, 2023 (in Thai)

Department of Marine and Coastal Resources, 2023, State of Marine and Coastal Resources and Coastal Erosion Thailand National Report 2021, 407 p. (in Thai)

Amaya, O., Quintanilla, R., Stacy, B.A., Dechraoui, B., M-Y., Flewelling, L., Hardy, R., Dueñas, C. and Ruiz, G., 2018, Large-Scale Sea Turtle Mortality Events in El Salvador Attributed to Paralytic Shellfish Toxin-Producing Algae Blooms, Frontiers in Marine Science, doi: 10.3389/fmars.2018.00411, 5: 1-10

Manire, C, A., Stacy, B., Flewelling, L., Rodriguez, M., Foley, A., Young, M. and Keene, J., 2016, Follow-Up Report on Unusual Strandings of Small Juvenile Green Sea Turtles (Chelonia mydas) in Southeast Florida, In IAAAM Conference Proceedings, International Association for Aquatic Animal Medicine

Hallegraeff, G.M., 2014, Harmful algae and their toxins: progress, paradoxes and paradigm shifts. In Rossini, G.P. (Ed.), Toxins and Biologically Active Compounds from Microalgae, Volume I: Origin, Chemistry and Detection, CRC Press, Boca Raton, p. 3-20

Kelchner, H.; Reeve-Arnold, K.E.; Schreiner, K.M.; Bargu, S.; Roques, K.G.; Errera, R.M., 2021. Domoic Acid and Pseudo-nitzschia spp. Connected to Coastal Upwelling along Coastal Inhambane Province, Mozambique: A New Area of Concern. Toxins 13, Available Sources:, March, 2023

Fire, E.F.,Andrea, B., Robert, A.D.,Greg, E.,Tod, A. L., Keith, M.,Glenn, A. M., Kathleen, M. T. M.,Michael, M., Misty, N., Katie, P., Zhihong, W., Frederick, W. W., 2021, An assessment of temporal, spatial and taxonomic trends in harmful algal toxin exposure in stranded marine mammals from the U.S. New England coast, Available Sources:, March, 2023

Oning, V., Shettapong, M., Nissara, T., Thaithaworn, L., 2017. Accumulation of domoic acid in marine organisms from Sriracha bay, Chonburi province, Thailand, ScienceAsia, 43 (4): 207-216

Shettapong, M., Nissara, T., Boontarika, T. and Oning, V., 2013. Study on Distribution of phytoplankton in the Central Gulf of Thailand. pp. 84-99., In Proceedings of 2013 Survey of Fisheries Resources and Marine Environment in the Central Gulf of Thailand by SEAFDEC Training Office Southeast Asian Fisheries Development Center, Samutprakarn (in Thai)

Fire, S.E., Wang, Z., Berman, M., Langlois, G.W., Morton, S.L., Sekula-Wood, E. and Benitez-Nelson, C.R., 2010. Trophic Transfer of the Harmful Algal Toxin Domoic Acid as a Cause of Death in a Minke Whale (Balaenoptera acutorostrata) Stranding in Southern California. Aquatic Mammals, 36 (4): 342-350

Fire, S., E., Wang, Z., Byrd, M., Whitehead, H.R., Paternoster, J and Morton,S.L., 2011. Co-occurrence of multiple classes of harmful algal toxins in bottlenose dolphins (Tursiops truncatus) stranding during an unusual mortality event in Texas, USA., Journal Harmful Algae, 10: 330-336

Twiner, M. J., Spencer, F., Lori, S., Leigh, D., Zhihong, W., Steve, M., Stephen, R., Brian, B., Teresa, K. R. and Randall, S. W., 2011. Concurrent Exposure of Bottlenose Dolphins (Tursiops truncatus) to Multiple Algal Toxins in Sarasota Bay, Florida, USA., PLOS ONE, 6, 7: 1-15

Bargu, S., Goldstein, T., Roberts, K., Li, C. and Gulland, F., 2012. Pseudo-nitzschia bloom, domoic acid, and related California sea lion stranding in Monterey Bay, California, Marine Mammal Science, 28 (2), 237-253

Charermchai, Y., C., Attawut, K. and Saroj, R., 2006, Relationships between water qualities and phytoplankton at Sriracha Bay, Chonburi province pp. 511-517., In Proceedings of 44th Kasetsart University Annual Conference: Fisheries, Kasetsart University, Bangkok (in Thai)

Thitima, N., Narumo,l K., Supanoi S. and Rachawadee C., 2019, Contamination of Biotoxin, Domoic Acid

in Stranded Marine Mammals in Thai Water, Burapha Science Journal, 24 (22): 550-567 (in Thai)

Heil, C.A. and Muni-Morgan, A.L., 2021, Florida’s Harmful Algal Bloom (HAB) Problem: Escalating Risks

to Human, Environmental and Economic Health with Climate Change, Frontiers in Ecology and Evolution,

Available Sourse:, March, 2023

Perrault, J. R., Perkins, C. R., Ajemian, M. J., Bresette, M. J., Mott, C. R., and Page-Karjian, A., 2020,

Harmful algal and cyanobacterial toxins in foraging green turtles (Chelonia mydas) in Florida's Big Bend,

Elsevier, Toxicon X, (5): 1-6

Quilliam, M.A., 2003, Chemical methods for domoic acid, the amnesic shellfish poisoning (ASP) toxin,

pp. 247-265, In Hallegraeff, G.M., Anderson D.M. and Cembella A.D. (Eds), Manual on Harmful Marine

Microalage, Monographs on Oceanographic Methodology, Vol II. Paris: Intergovernmental Oceanographic

Commission (UNESCO)

Law, R.J., Fileman, C. F., Hopkins, A.D., Barker, J.R.., Harwood, J., Jackson, D.B., Kennedy, S. Marin, A.R., and Morris, R.J., 1991, Concentration of trace metals in the livers of marine mammals (Seals, Porpoises and Dolphins) from waters around the British Isles, Marine Pollution Bullen 22(4): 183-191

Kemmper, C., Gibbs, P., Obendorf, D., Marvanek, S. and Lenghaus, C., 1994, A review of heavy metal and organochlorine levels in marine mammals in Australia, Science of the Total Environment, 154: 129-139

Geraci, J.R. and Lounsbury, V.J., 2005, Marine Mammals Ashore: A Field Guide for Stranding (second edition), Sparks Maryland, 309 p.

Angela, C., Leanne, J., Flewelling, K.A., 2013, Dietary exposure to harmful algal bloom (HAB) toxins in the endangered manatee (Trichechus manatus latirostris) and green sea turtle (Chelonia mydas) in Florida, USA., Harmful Algae, 28: 1-9

Stacy, B., Hardy, R., Shaver, D., Purvin, C., Howell, L., Wilson, H., Devlin, M., Krauss, A., Macon, C., Cook, M., Wang, Z., Flewelling, L., Keene, J., Walker, A., Baker, P. and Yaw, T., 2020, Sea Turtle Strandings in Texas: A Summary of Findings and Analyses. Department of Commerce, National Marine Fisheries Service, NOAA Technical Memorandum NMFS OPR-66, 64 p.

Thitima, N., 2010, Epiphytic Diatom on Seagrass Blade at Ban Pa Khlok, Phuket Province, Master of Science, Kasetsart University, Bangkok Thesis, 327 p. (in Thai)

Borowizka, M. A. Paul, S.L., and Michel V.K., 2006, Epiphytes of Seagrasses, In A.D.W. Larkum et al. (Eds), Biology, Ecology and Conservation, pp. 441-461, Springer

Sand-Jensen, K., 1977, Effect of epiphytes on eelgrass photosynthesis, Aquatic Botany, 3: 55-63

Jariyavadee, S. and Jittra, T., 2019, Factor effecting of nutrient on biomass and epiphytic community in seagrass bed, Sattahip bay, Chon Buri Province, Faculty of Science, Burapha University, 72 p. (in Thai)

Nipaporn, C., 1994. Biology and electron microscopic structure of the diatom Nitzschia sp. Master of Science, Kasetsart University, Bangkok, Thesis, 123 p. (in Thai)

Jackson, A.E., Stephen, W.A. and Maurice, V.L., 1992, The effect of salinity on growth and amino acid composition in the marine diatom Nitzschia pungens, Canadian Journal of Botany, 70(1): 2198-2201

Oning, V., 2017, Domoic acid contamination process in marine organisms: case on the east coast of the Gulf of Thailand, Doctor of Philosophy (Marine Science), Kasetsart University, Thesis, 201 p. (in Thai)

Priisholm, K., Moestrup, Ø. and Lundholm, N., 2011, Taxonomic notes on the marine diatom genus Pseudo-nitzschia in the Andaman Sea near the island of Phuket, Thailand, with a description of Pseudo-nitzschia micropora sp. nov., Diatom Research, 17(1): 153-175

Bates, S.S., 1998, Ecophysiology and Metabolism of ASP Toxin Production, Physiological ecology of harmful algal blooms, 405-426

Fehling, J., Green, D.H., Davidson, K., Bolch, C.J. and Bates, S.S., 2004, Domoic acid production by Pseudo-nitzschia seriata (Bacillariophyceae) in Scottish water, Journal Phycology, 40: 622-630

Liefer, J.D., Robertson, A., MacIntyre, H.L., Smith W.L. and Dorsey, C.P., 2013, Characterization of a toxic Pseudo-nitzschia spp. bloom in the Northern Gulf of Mexico associated with domoic acid accumulation in fish, Harmful Algae, 26: 20-32

Jennifer Maucher, F., Noah, M., Zhihong, W., John, S.R., 2012, Toxicokinetics of domoic acid in the fetal rat, Toxicology, 294 (1): 36-41

Landsberg, J.H., 2002, The effects of harmful algal blooms on aquatic organisms, Fisheries Science, 10: 113-390

Harris, H.S., Benson, S.R., Gilardi, K.V., Poppenga, R.H., Work, T.M., Dutton, P.H. and Mazet, J.AK., 2011, Comparative health assessment of Western Pacific leatherback turtles (Dermochelys coriacea) foraging off the coast of California, 2005-2007, Wildl Dis 47(2): 321-337

Harris, H.S., Benson, S.R., Miller, M.A., Fire, S.E., Stacy, B.A., Kudela, R.M., Fahy, C.C and Seminoff, J.A., 2013, Domoic Acid in the Leatherback Turtle Food Web on Critical Foraging Grounds in Central California, In Proceeding (2013) p. 2, International Association for Aquatic Animal Medicine