The Release of Silver Nanoparticles and Antibacterial Properties of Hydrogel Sheet Dressings
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Abstract
Recently theuseof silver nanoparticles (AgNPs) in wound dressings has attracted considerable attention due to their potent antibacterial properties. The aim of this research was to investigate the release of AgNPs from hydrogel dressings by inductively coupled plasma-mass spectrometry (ICP-MS) analysis and their antibacterial properties by disc diffusion assay.Freeze-thawing and electron beam (EB) irradiation techniques were used to crosslink hydrogel patches composed of silvernitrate atvaried concentrations. During EB irradiation silver nitrate was reduced to AgNPs within the hydrogel matrices. Although UV-Vis spectroscopy showed the characteristics absorbance peaked at 410 nm from all AgNPs-embedded hydrogels, the disc diffusion assay showed the formationof a clear zone
without bacteria only for hydrogels with AgNPs at the two highest concentrations, 0.5 and 1.0 mg/cm2 ,against both Staphylococcus aureusand Escherichia coli.The corresponding amounts of released AgNPs were ~ 65 and 225 ppm, respectively, as analyzed by ICP-MS. Taken together, the AgNPs-loaded hydrogels developed by freeze-thawing and EB irradiation techniques in this study have potential as antibacterial wound dressings
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