Effects of Cannabis sp. on Antioxidant and in vitroAnti-inflammatory Activities

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Published: Aug 15, 2024
Keywords:
Cannabis sp. Tetrahydrocannabinol Cannabidiol Cannabigerol Antioxidant Anti-inflammatory

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Chutima Kaewpiboon
Department of Biology, Faculty of Science and Digital Innovation, Thaksin University, Phatthalung 93210
Abdul-Wahab Salae
Department of Science and Mathematics, Faculty of Science, Phuket Rajabhat University, Phuket 83000
Nawong Boonnak
Department of Basic Science and Mathematics, Faculty of Science and Digital Innovation, Thaksin University, Songkhla 90000

Abstract

This research studied four cultivars of Cannabis sp. The results of the study classified the cultivars according to their cannabinoids composition of THC (tetrahydrocannabinol), CBD (cannabidiol) and CBG (cannabigerol) into four types (type I (THC dominant), type II (hybrid), type III (CBD dominant) and type IV (CBG dominant). These cultivars were the Thai stick, the King garden, the Charlotte’s angle, and the CBD zerodue, respectively. The total phenolic contents showed that the King garden cultivar had the highest level at 1033.60±9.42 mgGAE/g extract. The antioxidant activity, measured using the DPPH and ABTS assays, indicated that the King garden cultivar had the best antioxidant activity with EC50 values of 15.06±0.73 and 0.50±0.10 mg/mL, respectively. Moreover, it was found that the antioxidant activity was related to the total phenolic contents of each cultivar. The study of anti-inflammatory effects using protein denaturation inhibiting assay found that the CBG zerodue cultivar had the best effect, inhibiting protein denaturation by 57.51% at a concentration of 500 ug/mL.

Article Details

Issue
Vol.32 No.4 (July-August 2024)
Section
Biological Sciences

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