Efficiency of Thai Herbal Extracts from Thai Traditional Medicine Recipe Against Human Pathogenic Microorganisms and Antioxidant Activity
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Abstract
This research aims to evaluate the potential of extracts for antimicrobial and antioxidant activities from five medicinal plants used in a Thai traditional medicine recipe. Furthermore, the total phenolic and total flavonoids content were analyzed using the Folin Ciocalteu method and colorimetric aluminum chloride method, respectively. The five plants (Smilax corbularia, Smilax glabra, Stemona tuberosa, Melodorum siamense, Canna indica) were extracted with ethanol and ethyl acetate. Ten extracts were evaluated for their antimicrobial and antioxidant activities using a colorimetric broth microdilution test and DPPH radical scavenging assays, respectively. Ten human pathogenic microorganisms were used in the antimicrobial test: Staphylococcus aureus ATCC25923, Methicillin resistant Staphylococcus aureus (MRSA) SK1, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Candida albicans ATCC90028, Candida albicans NCPF3153, Cryptococcus neoformans ATCC90112, Cryptococcus neoformans ATCC90113, Microsporum gypseum and Taralomyces marneffei. The ethanol extract from M. siamense was the most active against P. aeruginosa and M. gypseum with minimum inhibitory concentration (MIC) of 0.4 mg/ml, followed by the ethanol extract from S. corbularia against S. aureus with an MIC of 0.8 mg/ml. The ethanol extract from S. corbularia had the strongest antioxidant activity (DPPH) with an IC50 of 3.08 ± 0.49 μg/ml. In addition, these active extracts were tested for total phenolic and total flavonoids content. They were found to contain high amount of total phenolics at 403.53 ± 0.83 mg of gallic acid equivalent/g extract and total flavonoids at 21.23±0.16 mg QE/g of extract. These results indicate that extracts from Thai herbals are promising as they are a good source of many active metabolites for future applications.
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References
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