Cannabinoids Extraction from Cannabis Cultivar Blueberry by Pulsed Electric Field
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Abstract
The aims of this study were to define the optimum conditions for pulsed electric field extraction of delta-9-tetrahydrocannabinol (THC) and total cannabinoids of the Cannabis sativa L. (Blueberry variety) and to apply the response surface method to design a Box Behnken experiment to investigate the effects of three independent variables: pulse numbers at levels 100-300, pulse intensity at 5-10 kV/cm, and the solution ratio at 1:20-1:40. The best yield result was then compared with the traditional maceration extraction method. The experiments found that the optimum conditions for extracting THC and total cannabinoids were 300 pulses, a pulse intensity of 7.5 kV/cm, and a solution ratio of 1:20. The maximum THC content was 254.77±11.01 mg/g, and the total cannabinoids content was 270.98±11.96 mg/g, which were higher than those obtained by maceration by 20.13% and 22.69%, respectively. Moreover, the extraction time was significantly shorter, by 88.9%. Therefore, the extraction of cannabinoids using the pulsed electric field technique can be an effective method for extracting high-value phytochemicals from cannabis, offering both efficiency and higher quality.
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