Erythromycin-Loaded Poly (Vinyl Alcohol)/Chitosan/Collagen Electrospun Fiber Mats as Antibacterial Wound Dressings
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Abstract
Electrospun fibers composed of poly (vinyl alcohol) (PVA), chitosan (CS), and collagen (CG) at different ratios and encapsulating erythromycin (EM) were fabricated using the electrospinning technique and crosslinked with glutaraldehyde for potential wound dressing applications. This study investigated the solution factors influencing fiber morphology, physical and chemical properties, EM release characteristics, antioxidant activity, and antibacterial properties. The obtained nanofibers were smooth and uniform, with diameters ranging from 164 to 218 nm. After crosslinking, the fiber diameters increased to a range of 182 to 228 nm. Contact angle measurements show higher hydrophilicity of the fiber mats with increasing CG content or decreasing PVA content, resulting in a higher degree of swelling and weight loss after immersion in a phosphate buffer solution (pH 7.4). The maximum cumulative release of EM ranged from 51.6% to 73.1%, while the maximum antioxidant activity ranged from 65% to 70%. Additionally, the electrospun fiber mats showed antibacterial activity against both Staphylococcus aureus and Escherichia coli. These findings indicate that the electrospun PVA/CS/CG – EM fiber mats possessed suitable properties for use as wound dressings
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