Effect of Sodium Hypochlorite in Culture Medium on Shoot Proliferation and Root Induction of Curcuma longa L.
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Abstract
The propagationof Curcuma longa L., a medicinal herb known for its therapeutic properties, including alleviating stomach discomfort and expelling gas, can be effectively supported through plant tissue culture to ensure an adequate supply for cultivation. However, the cost of autoclaving for media sterilization is high. The addition of sodium hypochlorite (NaOCl) to the medium can achievesterility and reduce cost, but it may also affect plant growth in vitro. Therefore, this study aimed to investigate the effect of various concentrations of NaOCl intheculture medium on shoot multiplication and root induction of C. longa under aseptic conditions. The in vitro shoots were cultured on Murashige and Skoog (MS) medium supplemented with 2 mg/L benzyladenine (BA) to induce shoot formation and on MS medium without plant growth regulators to induce root development. The culture medium was sterilized either by autoclaving (control) or by adding NaOCl at concentrations of 0.1, 0.2, 0.4, 0.6, and 0.8 mL/L. The results showed that all treatments effectively sterilized the culture medium. There were no significant differences among treatments in shoot and root induction percentage, number of shoots and roots, or shoot and root length. The survival rate after transplantation was 100%. Thus, NaOCl at a concentration of 0.1 mL/L can be effectively used for media sterilization in the micropropagation of C. longa.
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References
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