สารต้านจุลินทรีย์และสายสัมพันธ์เชิงวิวัฒนาการของราจากดินป่าชายเลน
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ม.ค. 3, 2018
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ราดิน ฤทธิ์ต้านจุลินทรีย์ สายสัมพันธ์เชิงวิวัฒนาการ ดินป่าชายเลน
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บทคัดย่อ
บทคัดย่อ
งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาศักยภาพของสารเมทาบอไลท์จากราดินในการต้านจุลินทรีย์และความสัมพันธ์เชิงวิวัฒนาการของราที่มีศักยภาพโดยเทคนิคทางชีวโมเลกุล แยกราจากดินป่าชายเลนได้ทั้งหมด 106 ไอโซเลท นำมาเลี้ยงในอาหาร potato dextrose broth ที่อุณหภูมิห้องโดยไม่เขย่าเป็นเวลา 3 สัปดาห์ น้ำเลี้ยงราสกัดด้วยเอทิลอะซิเตท (broth ethyl acetate, BE) และเส้นใยราสกัดด้วยเอทิลอะซิเตท (cell ethyl acetate, CE) และเฮกเซน (cell hexane, CH) สารสกัด 318 สาร นำมาทดสอบฤทธิ์ต้านจุลินทรีย์โดยวิธี colorimetric broth microdilution พบสารสกัด 8 สาร จากรา 6 ไอโซเลท มีศักยภาพสูงสุดในการยับยั้ง Staphylococcus aureus และ methicillin-resistant Staphylococcus aureus (MRSA) โดยสารที่มีศักยภาพสูงสุด คือ BE จาก TSU-MF1 (Talaromyces flavus) และ CE จาก TSU-MF2 (Penicillium sp.) และ TSU-MF5 (Xylaria feejeensis) มีฤทธิ์ยับยั้ง MRSA ได้ดีที่สุดให้ค่า MIC/MBC เท่ากับ 64/128 ไมโครกรัมต่อมิลลิลิตรจัดจำแนกรา 6 ไอโซเลท โดยข้อมูลลำดับนิวคลีโอไทด์ของดีเอ็นเอบริเวณ ITS หรือ 28S ไรโบโซมอลดีเอ็นเอ พบรา 5 ไอโซเลท มีความใกล้ชิดกับไฟลัมแอสโคไมโคตา (Talaromyces flavus, Penicillium sp., Hongkongmyces pedis, Dothidiomycetes sp. และ Xylaria feejeensis) และไฟลัมเบสิดิโอไมโคตา 1 ไอโซเลท (Subulicystidium sp.) จากผลการศึกษายืนยันว่าราดินมีความหลากหลายของชนิดพันธุ์ และมีศักยภาพในการผลิตสารต้านจุลินทรีย์
คำสำคัญ : ราดิน; ฤทธิ์ต้านจุลินทรีย์; สายสัมพันธ์เชิงวิวัฒนาการ; ดินป่าชายเลน
Article Details
ประเภทบทความ
Biological Sciences
เอกสารอ้างอิง
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[18] Nylander, J.A.A., 2004, MrModeltest v. 2.0, Evolutionary Biology Centre, Uppsala University, Sweden.
[19] Borwornwiriyapan, K., 2013, Screening of soil fungi from plant genetic conservation project area, Rajjaprabha dam, Suratthani province which produced antimicrobial substances. M.Sc. Thesis, Prince of Songkla University, Songkhla.
[20] Standing, D. and Killham, K., 2007, The Soil Environment, pp. 1-22, Elsas, J.D.V., Jansson, J.K. and Trevors, J.T. (Eds.), Modern Soil Microbiology, 2nd Ed., CRC press, Boca Raton.
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[22] Rao, V.V.P. and Brahmaji, R. P., 2014, Physico-Chemical analysis of mangrove soil in the Machilipatnam coastal region, Krishna district, Andhra Pradesh, IJERT. 3: 10-12.
[23] Das, S., De, M., Ray, R., Ganguly, D., Jana, T.K. and De, T.K., 2011, Salt tolerant culturable microbes accessible in the soil of the Sundarban mangrove forest, India Open Ecol. J. 2: 35-40.
[24] Mazumder, P.M., Mazumder, R., Mazumder, A. and Sasmal, D.S., 2002, Antimicrobial activity of the mycotoxin citrinin obtained from the fungus Penicillium citrinum, Anc. Sci. Life 3: 191-197.
[25] Thatoi, H., Behere, B.C., Dangar, T.K. and Mishra, R.R., 2010, Microbial biodiversity in mangrove soils of Bhitarkanika, Odisha, India Int. J. Environ. Biol. 2: 50-58.
[26] Sahoo, K. and Dhal, N.K., 2009, Potential microbial diversity in mangrove eco-systems: A review, Indian J. Mar. Sci. 38: 249-256.
[27] Wongthong, S., Bangrak, P., Phongpaichit, S., Somrithpol, S. and Songkumarn, P., 2014. Antimicrobial activity of soil fungi from Khao Nan National Park, Nakhon Si Thammarat province, Thailand, JPAM. 8: 2999-3010.
[28] Elias, B.C., Said, S., de Albuquerque, S. and Pupo, M.T., 2006, The influence of culture conditions on the biosynthesis of secondary metabolites by Penicillium verrucosum Dierck, Microbiol. Res. 161: 273-280.
[29] Talukdar, S., Talukdar, M., Buragohian, M., Yadav, A., Yadav, R.N.S. and Bora, T.C., 2016, Enhanced candicidal compound production by a new soil isolate Penicillium verruculosum MKH7 under submerged fermentation, BMC. Microbiol. 16: 1-12.
[30] Lihan, S., Lin, C.D., Ahmad, I., Sinang, F.M., Hua, N.K. and Sallehin, A.A., 2014, Antimicrobial producing microbes isolated from soil sample collected from Nanga Merit Forest in Sarawak, Malaysian Borneo, Euro. J. Exp. Bio. 4: 494-501.
[31] Khalil, A.M.A., El-sheikh, H.H. and Sultan, M.H., 2013, Distribution of fungi in mangrove soil of coastal areas at Nabq and Ras Mohammed Protectorates, J. Curr. Microbiol. Appl. Sci. 2: 264-274.
[32] Tsang, C.C.C., Chan, J.F.W., Trendell-Sm, N.J., Ngan, A.H.Y., Ling, I.W.H., Yuen, K.Y., Lau, S.K.P. and Wood, P.C.Y., 2014, Subcutaneous phaeohyphomycosis in a patient with IgG4-related sclerosing disease caused by a novel ascomycete, Hongkongmyces pedis gen. et sp. nov.: First report of human infection associated with the family Lindgomycetaceae, Med. Mycol. 52: 736-747.
[33] Thorn, R.G., Reddy, C.A., Harris, D. and Pual, E.A., 1996, Isolation of saprophytic Basidiomycetes from soil, Appl. Environ. Microbiol. 62: 4288-4292.
[2] Hawksworth, D.L., 2001, The magnitude of fungal diversity: The 1.5 million species eatimate revisited, Mycol. Res. 105: 1422-1432.
[3] Livermore, D.M., 2011, Discover research: The scientific challenge of finding new antibiotics, J. Antimicrob. Chemother. 66: 1941-1944.
[4] Monton, J.B., 2005, Fungi, pp. 141-161, Yarnell, D., Ed., Principles and application of soil microbiology, 2nd Ed., Pearson Education, Inc., New Jersey.
[5] Cazar, M.E., Schmeda-Hirschmann, G. and Astudillo, L., 2005, Antimicrobial butylolactone I derivatives from the Ecuadorain soil fungus Aspergillus terreus Thorn. Var terreus, World J. Microbiol. Biotechnol. 21: 1067-1075.
[6] Xioa-Yan, S., Qing-Tao, S., Shu-Tao, X., Xiu-Lan, C., Cai-Yun, S. and Yu-Zhong, Z., 2006, Broad-spectrum antimicrobial activity and high stability of trichokonins from Trichoderma koningii SMF2 against plant pathogens, FEMS Microbiol. Rev. 260; 119-125.
[7] Gharaei-Fathabad, E., Tajick-Ghanbary, M. A. and Shahronkri, N., 2009, Antimicrobial properties of Penicillium species isolated from agriculture soils of Northern Iran, Res. J. Toxins 1: 1-7.
[8] Jachowshi, N.R.A., Quak, M.S.Y., Friess, D.A., Duangnamon, D., Webb, E.L. and Ziegler, A.D., 2013, Mangrove biomass estimation in Southwest Thailand using machine learning, Appl. Geogr. 45: 311-321.
[9] Barnett, H.L. and Hunter, B.B., 1998, Illustrated Genera of Imperfect Fungi, Prentice Hall, Inc., New York.
[10] Clinical and Laboratory Standards Institute (CLSI), 2002a, Reference method for broth dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved standard M7-A4.Clinical and Laboratory Standards Institute, Wayne, Pa.
[11] Clinical and Laboratory Standards Institute (CLSI), 2002b, Reference method for broth dilution antimicrobial susceptibility testing of yeasts.Approved standard M27-A2, Clinical and Laboratory Standards Institute, Wayne, Pa.
[12] O'Donnell, K., Cigelnik, E., Weber, N.S. and Trappe, J.M., 1997, Phylogenetic relationships among Ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis, Mycologia 89: 48-65.
[13] Hall, T., 2005, BioEdit: Biological Sequence Alignment Editor for Windows 95/98/NT/ XP, Available: http://www.mbio.ncsu.edu/ bioedit/page1.html.
[14] Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and wwight matrix choice, Nucl. Acids Res. 22: 4673-4680.
[15] Swofford, D.L., 2002. PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4, Sinauer Associates, Sunderland, Massachusetts.
[16] Kishino, H. and Hasegawa, M., 1989, Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea, J. Mol. Evol. 29: 170-179.
[17] Huelsenbeck, J.P. and Ronquist, F., 2001, MrBayes: Bayesian inference of phylo-genetic trees, Bioinformatics 17: 754-755.
[18] Nylander, J.A.A., 2004, MrModeltest v. 2.0, Evolutionary Biology Centre, Uppsala University, Sweden.
[19] Borwornwiriyapan, K., 2013, Screening of soil fungi from plant genetic conservation project area, Rajjaprabha dam, Suratthani province which produced antimicrobial substances. M.Sc. Thesis, Prince of Songkla University, Songkhla.
[20] Standing, D. and Killham, K., 2007, The Soil Environment, pp. 1-22, Elsas, J.D.V., Jansson, J.K. and Trevors, J.T. (Eds.), Modern Soil Microbiology, 2nd Ed., CRC press, Boca Raton.
[21] Yongchalermchai, C., Nilnond, C. and Pechkep, S., 2011, A study of diversity of soils and morphology with their chemical and physical properties, The complete research report, Prince of Songkla University, Songkhla.
[22] Rao, V.V.P. and Brahmaji, R. P., 2014, Physico-Chemical analysis of mangrove soil in the Machilipatnam coastal region, Krishna district, Andhra Pradesh, IJERT. 3: 10-12.
[23] Das, S., De, M., Ray, R., Ganguly, D., Jana, T.K. and De, T.K., 2011, Salt tolerant culturable microbes accessible in the soil of the Sundarban mangrove forest, India Open Ecol. J. 2: 35-40.
[24] Mazumder, P.M., Mazumder, R., Mazumder, A. and Sasmal, D.S., 2002, Antimicrobial activity of the mycotoxin citrinin obtained from the fungus Penicillium citrinum, Anc. Sci. Life 3: 191-197.
[25] Thatoi, H., Behere, B.C., Dangar, T.K. and Mishra, R.R., 2010, Microbial biodiversity in mangrove soils of Bhitarkanika, Odisha, India Int. J. Environ. Biol. 2: 50-58.
[26] Sahoo, K. and Dhal, N.K., 2009, Potential microbial diversity in mangrove eco-systems: A review, Indian J. Mar. Sci. 38: 249-256.
[27] Wongthong, S., Bangrak, P., Phongpaichit, S., Somrithpol, S. and Songkumarn, P., 2014. Antimicrobial activity of soil fungi from Khao Nan National Park, Nakhon Si Thammarat province, Thailand, JPAM. 8: 2999-3010.
[28] Elias, B.C., Said, S., de Albuquerque, S. and Pupo, M.T., 2006, The influence of culture conditions on the biosynthesis of secondary metabolites by Penicillium verrucosum Dierck, Microbiol. Res. 161: 273-280.
[29] Talukdar, S., Talukdar, M., Buragohian, M., Yadav, A., Yadav, R.N.S. and Bora, T.C., 2016, Enhanced candicidal compound production by a new soil isolate Penicillium verruculosum MKH7 under submerged fermentation, BMC. Microbiol. 16: 1-12.
[30] Lihan, S., Lin, C.D., Ahmad, I., Sinang, F.M., Hua, N.K. and Sallehin, A.A., 2014, Antimicrobial producing microbes isolated from soil sample collected from Nanga Merit Forest in Sarawak, Malaysian Borneo, Euro. J. Exp. Bio. 4: 494-501.
[31] Khalil, A.M.A., El-sheikh, H.H. and Sultan, M.H., 2013, Distribution of fungi in mangrove soil of coastal areas at Nabq and Ras Mohammed Protectorates, J. Curr. Microbiol. Appl. Sci. 2: 264-274.
[32] Tsang, C.C.C., Chan, J.F.W., Trendell-Sm, N.J., Ngan, A.H.Y., Ling, I.W.H., Yuen, K.Y., Lau, S.K.P. and Wood, P.C.Y., 2014, Subcutaneous phaeohyphomycosis in a patient with IgG4-related sclerosing disease caused by a novel ascomycete, Hongkongmyces pedis gen. et sp. nov.: First report of human infection associated with the family Lindgomycetaceae, Med. Mycol. 52: 736-747.
[33] Thorn, R.G., Reddy, C.A., Harris, D. and Pual, E.A., 1996, Isolation of saprophytic Basidiomycetes from soil, Appl. Environ. Microbiol. 62: 4288-4292.
