Effect of Protein Source from Dried Black Soldier Fly (Hermetia illucens) on Egg Quality in Commercial Laying Hens Raised in Opened–house
Main Article Content
Abstract
The larvae of black soldier fly contain a large amount of protein. They might thus be incorporated into laying hen diets as a source of protein. Therefore, the objective of this research was to study the effect of protein source from dried black soldier fly larvae on egg quality in twenty-nine weeks old of Lohmann Brown laying hens. A hundred hens were divided into four groups with twenty-five replications. Completely randomize design was used. Laying hens were fed diets with four different protein sources: fish meal and soybean meal (FSH), 100% soybean meal (SOY), 2% black soldier fly with soybean meal (BS2) and 5% black soldier fly with soybean meal (BS5). Egg quality traits were egg weight (ETW), albumin weight (ALW), yolk weight (YKW), shell weight (SHW), Haugh unit (HU), yolk color score (YCS), shell thickness at tip (STT), shell thickness at middle (STM) and shell thickness at base (STB). The results found that type of protein source had no statistically significant difference on ETW, ALW, YKW, SHW, HU and YCS. Least squares mean for ETW, ALW, YKW, SHW, HU and YCS ranged from 49.98 (BS5) to 50.91 g (SOY), 32.64 (BS5) to 33.78 g (SOY), 11.86 (FSH) to 12.00 g (BS5), 5.05 (SOY) to 5.35 g (BS5), 84.58 (BS5) to 84.74 unit (FSH), and 3.24 (SOY) to 3.63 unit (BS2), respectively. Feed costs for FSH, SOY, BS2 and BS5 were 15.16, 15.76, 14.79 and 13.70 baht per kilogram. According to this study, 5% BSF with soybean meal could be fed the laying hens without harming their egg qualities and this diet was also more affordable than other feed formulations.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Al-Qazzaz, M.F.A., D. Ismail, H. Akit and L.H. Idris. 2016. Effect of using insect larvae meal as a complete protein source on quality and productivity characteristics of laying hens. R. Bras. Zootec. 45(9): 518–523.
Anankware, P.J., R.A. Ayizanga, O. Opoku and D. Obeng-Ofori. 2018. Potential of the black soldier fly (Hermetia illuscens) as a replacement for fish/soybean meal in the diet of broilers. Glo. Adv. Res. J. Agric. Sci. 7(8): 272–280.
Attivi, K., K. Agboka, G.K. Mlaga, O.E. Oke, A. Teteh, O. Onagbesan and K. Tona. 2020. Effect of black soldier fly (Hermetia illucens) maggots meal as a substitute for fish meal on growth performance, biochemical parameters and digestibility of broiler chickens. Int. J. Poult. Sci. 19(2): 75–80.
Barragan-Fonseca, K.B., M. Dicke and J.J.A. van Loon. 2017. Nutritional value of the black soldier fly (Hermetia illucens L.) and its suitability as animal feed – a review. J. Insects Food Feed. 3(2): 105–120.
Barrett, N.W., K. Rowland, C.J. Schmidt, S.J. Lamont, M.F. Rothschild, C.M. Ashwell and M.E. Persia. 2019. Effect of acute and chronic heat stress on the performance, egg quality, body temperature, and blood gas parameters of laying hens. Poult. Sci. 98(12): 6684–6692.
Bejaei, M. and K.M. Cheng. 2020. The effect of including full-fat dried black soldier fly larvae in laying hen diet on egg quality and sensory characteristics. J. Insects Food Feed 6(3): 305–314.
Berman, A., T. Horovitz, M. Kaim and H. Gacitua. 2016. A comparison of THI indices leads to a sensible heat–based heat stress index for shaded cattle that aligns temperature and humidity stress. Int. J. Biometeorol. 60(10): 1453–1462.
Bessa, L.W., E. Pieterse, J. Marais and L.C. Hoffman. 2020. Why for feed and not for human consumption? The black soldier fly larvae. Compr. Rev. Food Sci. Food Saf. 19(5): 2747–2763.
Bondari, K. and D.C. Sheppard. 1981. Soldier fly larvae as feed in commercial fish production. Aquaculture 24: 103–109.
Bryden, W.L., X. Li, I. Ruhnke, D. Zhang and S. Shini. 2021. Nutrition, feeding and laying hen welfare. Anim. Prod. Sci. 61: 893–914.
Burana, K. and T. Jamjanya. 2010. Distribution, rearing methods and nutritional value of black soldier fly (Hermetia illucens L.), pp. 603–609. In Proc. the 11th Graduate Research Conference Khon Kaen University, 12 February 2010. (in Thai)
Chia, S.Y., C.M. Tanga, I.M. Osuga, A.O. Alaru, D.M. Mwangi, M. Githinji, T. Dubois, S. Ekesi, J.J.A. van Loon and M. Dicke. 2021. Black soldier fly larval meal in feed enhances growth performance carcass yield and meat quality of finishing pigs. J. Insects Food Feed. 7(4): 433–447.
Cullere, M., G. Tasoniero, V. Giaccone, R. Miotti-Scapin, E. Claeys, S. De Smet and A. Dalle Zotte. 2016. Black soldier fly as dietary protein source for broiler quails: apparent digestibility, excreta microbial load, feed choice, performance, carcass and meat traits. Animal 10(12): 1923–1930.
Dalle Zotte, A., Y. Singh, J. Michiels and M. Cullere. 2019. Black soldier fly (Hermetia illucens) as dietary source for laying quails: live performance, and egg physico-chemical quality, sensory profile and storage stability. Animals 9(3): 115.
Etches, R.J., T.M. John and A.M.V. Gibbins. 2008. Behavioural, physiological, neuroendocrine and molecular responses to heat stress, pp. 48–79. In N. Daghir, ed. Poultry Production in Hot Climates. 2nd edition. CABI, Oxfordshire, UK.
Khempaka, S., K. Koh and Y. Karasawa. 2006. Effect of shrimp meal on growth performance and digestibility in growing broilers. J. Poult. Sci. 43(3): 250–254.
Kilic, I. and E. Simsek. 2013. The effects of heat stress on egg production and quality of laying hens. J. Anim. Vet. Adv. 12(1): 42–47.
Kim, D.H., Y.K. Lee, S.H. Kim and K.W. Lee. 2021. The impact of temperature and humidity on the performance and physiology of laying hens. Animals 11(1): 56.
Lalander, C., S. Diener, C. Zurbrügg and B. Vinneras. 2019. Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). J. Clean. Prod. 208: 211–219.
Marono, S., R. Loponte, P. Lombardi, G. Vassalotti, M.E. Pero, F. Russo, L. Gasco, G. Parisi, G. Piccolo, S. Nizza, C. Di Meo, Y.A. Attia and F. Bovera. 2017. Productive performance and blood profiles of laying hens fed Hermetia illucens larvae meal as total replacement of soybean meal from 24 to 45 weeks of age. Poult. Sci. 96(6): 1783–1790.
Mashaly, M.M., G.L. Hendricks, M.A. Kalama, A.E. Gehad, A.O. Abbas and P.H. Patterson. 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83(6): 889–894.
National Research Council (NRC). 1994. Nutrient Requirements of Poultry. 9th edition. National Academy Press, Washington, D.C., USA.
Nguyen, T.T.X., J.K. Tomberlin and S. Vanlaerhoven. 2015. Ability of black soldier fly (Diptera: Stratiomyidae) larvae to recycle food waste. Environ. Entomol. 44(2): 406–410.
Ruhnke, I., C. Normant, D.L.M. Campbell, Z. Iqbal, C. Lee, G.N. Hinch and J. Roberts. 2018. Impact of on-range choice feeding with black soldier fly larvae (Hermetia illucens) on flock performance, egg quality, and range use of free-range laying hens. Anim. Nutr. 4(4): 452–460.
Secci, G., F. Bovera, S. Nizza, N. Baronti, L. Gasco, G. Conte, A. Serra, A. Bonelli and G. Parisi. 2018. Quality of eggs from Lohmann Brown Classic laying hens fed black soldier fly meal as substitute for soya bean. Animal 12(10): 2191–2197.
Senkwankaew, C., P. Khenjan, P. Sooksoi and N. Thongtip. 2017. Using of chitosan on growth performance in swine. Prawarun Agr. J. 14(2): 136–145. (in Thai)
Sripontan, Y., T. Juntavimon, S. Songin and C.I. Chiu. 2017. Egg-trapping of black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) with various wastes and the effects of environmental factors on egg-laying. Khon Kaen Agr. J. 45(1): 179–184.
Tancho, A. 2017. Maejo Maggots. Trio Advertising & Media Co., Ltd., Chiang Mai, Thailand. 90 pp. (in Thai)
Wang, Y.S. and M. Shelomi. 2017. Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods 6(10): 91.