Gamma Ray Induced Mutation in Chrysanthemum and Detection of DNA polymorphism by AFLP

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Published: Dec 31, 2017
Keywords:
Chrysanthemum mutation gamma ray AFLP

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Pantipa Limsanguan
Center for Agricultural Biotechnology, Kasetsart University and Center of Excellence on Agricultural Biotechnology: (AG–BIO/PERDO–CHE), Bangkok 10900, Thailand
Sontichai Chanprame
Center for Agricultural Biotechnology, Kasetsart University and Center of Excellence on Agricultural Biotechnology: (AG–BIO/PERDO–CHE), Bangkok 10900, Thailand; Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand
Ittirit Ungvichian
Thailand Institute of Scientific and Technological Research, Khlong Luang, Pathum Thani 12120, Thailand
Pattama Srinamnguen
Faculty of Science and Arts, Burapha University, Chanthaburi Campus, Chanthaburi 22170, Thailand
Sermsiri Chanprame
Center for Agricultural Biotechnology, Kasetsart University and Center of Excellence on Agricultural Biotechnology: (AG–BIO/PERDO–CHE), Bangkok 10900, Thailand; Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University,Nakhon Pathom 73140, Thailand

Abstract

The in vitro node explants of available commercial bicolor spray chrysanthemum cut flower cultivar (unknown cultivar’s name) were acute irradiated with 0, 5, 10, 15, 20, 25 and 30 Gy gamma ray. The irradiated nodes were then cultured on solid MS medium.The higher dose of gamma irradiation, the lower survival percentage obtained. The survival percentage of 5 and 10 Gy irradiated nodes were 100% while the lowest survival percentage of 3.3% obtained from 30 Gy. Therefore, the LD50 at 30 days after irradiation was 26 Gy. After transplanting in the grower farm, the normal growth and normal plant characters of all plantlets were observed. Change in flower color was observed in the dosages of 15, 20 and 25 Gy. The polymorphism also observed from AFLP using 10 primer pairs and as the irradiation dosage increased, the higher polymorphic bands observed. The dendrogram of the polymorphism revealed the separation of chrysanthemum between normal flowers and those color mutants.

Article Details

Issue
Vol. 48 No. 3 (2017): September − December
Section
Research article