Effect of Gamma Irradiation on Hybrid Ornamental Sweet Potato (Ipomoea batatas L.) In vitro

Abstract

The ornamental sweet potato is famous at present. It could be well-adjusted to environmental stresses and encounters drought stress. In ornamental world trade, there is little diversity of ornamental sweet potato. In this study, the objective was to study the effect of gamma irradiation for variation from mutation on hybrid ornamental sweet potato in vitro. In this experiment, the axillary buds of ornamental sweet potatoes, lobe leaf (MTL) and deep lobe leaf (MTDL) cultivars, were used for explants in clean culture. After 8 weeks of culturing on MS medium, they were subcultured and treated with acute doses of gamma-ray, 20, 40, 60, 80 and 100 gray, respectively. About 60 days after irradiations, LD50(60d) were calculated. The LD50(60d) of MTL was 30.001 Gy and that of MTDL was 46.665 Gy. After that, they were subcultured until M1V3 generation and transferred into greenhouse for cropping. The 20 gray of MTDL had color change, the light green of leaves, those differed from the non-irradiation one.