Identification and Chemical Control of Rhizoctonia species causing Sheath Bight Disease of Rice
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Abstract
The purpose of this were to study morphology and evaluate the efficiency of fungicides to control Rhizoctonia causing sheath rice disease. Diseased rice samples were collected from the north of Thailand. Fungi were isolated by tissue transplanting method and were identified base on morphological characteristics including number of nucleus, color, size of mycelial and sclerotia characteristic. Species identification was confirmed by molecular technique and PCR was conducted at internal transcribed spacer (ITS) rRNA gene region. Eighteen isolates of Rhizoctonia solani, 2 isolates of R. oryzae and 34 isolates of R. oryzae–sativae were identified and were confirmed as causal agent of sheath disease of rice. Six fungicide treatments, including azoxystrobin + tebuconazole, carbendazim, difenoconazole, mancozeb, prochloraz + propiconazole, and validamycin were evaluated for the inhibition of the fungal mycelium growth. The results demonstrated that growth of R. oryzae-sativae and R. solani inhibited by carbendazim and azoxystrobin + tebuconazole at 100%, 100% and 85.06% and 80.00%, respectively, while mancozeb and azoxystrobin + tebuconazole inhibited R. oryzae at 100% and 90.82%, respectively. In vivo experiments, azoxystrobin + tebuconazole exhibited strongest control of sheath rice disease caused by R. solani, R. oryzae and R. oryzae–sativae.
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References
Agrios, G. 2005. Plant Pathology. 5th edition. Elsevier Academic Press, USA. 922 pp.
Datta, S., P. Dey, A. Sarkar, J. Tarafdar and A. Chowdhury. 2016. Comparison among four triazole fungicides on growth and development of sheath blight of rice pathogen Rhizoctonia solani Kühn AG1–1A. Arch. Phytopathol. Plant Prot. 49: 239–251.
Department of Agriculture. 2016. Index of Plant Disease in Thailand. Department of Agriculture, Plant Pathology Research Group, Plant Protection Research and Development Office. 280 pp. (in Thai)
Disataporn, S. 1982. Rice Disease and Prevention. Department of Agriculture, Plant Pathology Research Group, Plant Protection Research and Development Office. 48 pp. (in Thai)
El–Shafey, R.A., R.M. Elamawi, M.M. Saleh, A.M. Tahoon and A.A. Emeran. 2019. Morphological, pathological and molecular characterization of rice sheath blight disease causal organism Rhizoctonia solani AG–1 IA in Egypt. Arch. Phytopathol. Plant Prot. 52: 507–529.
Groth, D.E. and J.A. Bond. 2007. Effects of cultivars and fungicides on rice sheath blight, yield, and quality. Plant Dis. 91: 1647–1650.
Kim, W.G. 1993. Morphological and cultural characteristics of fungi causing rice sclerotial diseases. Kr. J. Mycol. 21: 16–22.
Kumar, V., V.P. Chaudhary, D. Kumar, A. Kumar, S. Sagar and S. Chaudhary. 2017. Efficacy of botanicals and fungicides against Rhizoctonia solani inciting sheath blight disease on rice (Oryza sativa L.). J. Appl. Nat. Sci.9: 1916–1920.
Lanoiselet, V.M., G.J. Ash, E.J. Cother, M.J. Priest and A. Watson. 2001. First report of Waitea circinate causing sheath spot and Rhizoctonia oryzae–sativae causing aggregate sheath spot on rice in south–eastern Australia. Australas. Plant Pathol. 30: 369–370.
Lanoiselet, V.L., E.J. Cother, G.J. Ash and J.D.I. Harper. 2005. Yield loss in rice caused by Rhizoctonia oryzae and R. oryzae– sativae in Australia. Australas. Plant Pathol. 34: 175–179.
Lanoiselet, V.M., E.J. Cother and G.J. Ash. 2007. Aggregate sheath spot and sheath spot of rice. Crop Prot. 26: 799–808.
Mughal, M.N., S. Bashir, N.A. Bhat and K.A. Bhat. 2017. Cultural and morphological variability and identification of anastomosis group of Rhizoctonia solani (Thanatephorus cucumeris) causing sheath blight of rice in Kashmir. Int. J. Curr. Microbiol. Appl. Sci. 6: 3787–3794.
Nagaraj, B.T., G. Sunkad, P. Devenna, M.K. Naik and M.B. Patil. 2019. Characterization of Rhizoctonia species complex associated with rice sheath disease in Karnataka. Agric. Res. 8: 191–196.
Okubara, P.A., K.L. Schroeder and T.C. Paulitz. 2008. Identification and quantification of Rhizoctonia solani and R. oryzae using real–time polymerase chain reaction. Phytopathology 98: 837–847.
Ou, S.H. 1985. Rice Diseases. 2nd edition. Commonwealth Mycological Institute, Philippines. 380 pp.
Park, D.S., R.J. Sayler, Y.G. Hong, M.H. Nam and Y. Yang. 2008. A method for inoculation and evaluation of rice sheath blight disease. Plant Dis. 92: 25–29.
Persaud, R., A. Khan, W.A. Isaac, W. Ganpat and D. Saravanakumar. 2019. Plant extracts, bioagents and new generation fungicides in the control of rice sheath blight in Guyana. Crop Prot. 119: 30–37.
Pralhad, S.P., P.U. Krishnaraj and S.K. Prashanthi. 2019. Morphological and molecular characterization of Rhizoctonia solani causing sheath blight in rice. Int. J. Curr. Microbiol. Appl. Sci. 8: 1714–1721.
Pramesh, D., Maruti, A. Saddamhusen, K.M. Muniraju and G.S. Guruprasad. 2016. A new combination fungicide active ingredients for management of sheath blight disease of paddy. Adv. Res. 8: 1–7.
Than, P., R. Jeewon, K.D. Hyde, S. Pongsupasamit, O. Mongkolporn and P.W.J. Taylor. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol. 57: 562–572.
White, T.J., T. Bruns, S. Lee and J.W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315–322. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.L. White, eds. PCR Protocols: A Guide to Methods Applications. Academic Press, San Diego, CA, USA.
