Development and Validation of an Ultra Performance Liquid Chromatography Photodiode Array Method for Quantification of Low Levels of Triamcinolone Acetonide in Skin Permeation Studies
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Abstract
An analytical method using ultra-performance liquid chromatography coupled with photodiode array (UPLC-PDA) was developed and validated to quantify triamcinolone acetonide (TA) at a low level in samples from skin permeation studies which were based in phosphate buffer solution. Dexamethasone was used as an internal standard (IS) for evaluation. The separation of analytes and IS was performed on an ACQUITY UPLCTM BEH Shield RP18 column (1.7 μm, 100 mm x 2.1 mm I.D.) with a gradient system using 0.05% trifluoroacetic acid and acetonitrile as mobile phases with 5 min total run time and a detectable wavelength of 242 nm for a maximum absorbance of TA. Calibration curve linearity was linear over concentrations ranging from 50 ng/mL to 1,000 ng/mL with a correlation coefficient (r2) over 0.998. The lower limit of quantification (LLOQ) for TA was also as low as 50 ng/mL. Accuracy, precision, stability, and dilution integrity were evaluated to be within the acceptance criteria. Therefore, this newly developed UPLC-PDA method of high sensitivity and accuracy is promising for TA determination in samples from in vitro permeation experiments for dermatopharmacokinetic studies.
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