Anti-Proliferative and Apoptotic Effects of Ethanolic Extracts of the Seaweed Caulerpa lentillifera Against Human Glioblastoma Cells

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Kant Sangpairoj
Pornpun Vivithanaporn
Tanapan Siangcham


Caulerpa lentillifera (CL), or sea grapes, is a widely consumed as food in Asia. Our previous study showed the anti-proliferative and apoptosis effects of the hexane extract of this seaweed. We investigated the effects of the ethanolic extract of this seaweed (CLET) on the in vitro proliferation and apoptotic cell death in A172 human glioblastoma cells using MTT, CFSE, and Annexin V-FITC assays.  CLET at 500 µg/ml reduced A172 cell viability by inducing apoptotic cell death, while lower concentrations of CLET reduced proliferation of A172 cells. The present study suggests that CLET could inhibit proliferation and promote apoptotic cell death in human GBM cells, suggesting its potential to be used as an alternative treatment for glioblastoma.


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Mohamed S, Hashim SN, Rahman HA. Seaweeds: A sustainable functional food for complementary and alternative therapy. Trends Food Sci Technol. 2012;23(2):83-96.

Gutierrez-Rodriguez AG, Juarez-Portilla C, Olivares-Banuelos T, Zepeda RC. Anticancer activity of seaweeds. Drug Discov Today. 2018;23(2):434-447.

Moussavou G, Kwak DH, Obiang-Obonou BW, Maranguy CA, Dinzouna-Boutamba SD, Lee DH, et al. Anticancer effects of different seaweeds on human colon and breast cancers. Mar Drugs. 2014;12(9):4898-4911.

Ratana-Arporn P, Chirapart A. Nutritional evaluation of tropical green seaweeds Caulerpa lentillifera and Ulva reticulata. Agr Nat Resour. 2006;40(6):75-83.

Osotprasit S, Samrit T, Chaiwichien A, Changklungmoa N, Meemon K, Niamnont N, et al. Toxicity and anti-oxidation capacity of the extracts from Caulerpa lentillifera. CMUJ Nat Sci. 2021;20(3):e2021065.

Yoojam S, Ontawong A, Lailerd N, Mengamphan K, Amornlerdpison D. The enhancing immune response and anti-inflammatory effects of Caulerpa lentillifera extract in RAW 264.7 cells. Molecules. 2021;26(19):5734.

Yao M, Li S, Wu X, Diao S, Zhang G, He H, et al. Cellular origin of glioblastoma and its implication in precision therapy. Cell Mol Immunol. 2018;15(8):737-739.

Mehra R, Bhushan S, Bast F, Singh S. Marine macroalga Caulerpa: role of its metabolites in modulating cancer signaling. Mol Biol Rep. 2019;46(3):3545-3555.

Tanawoot V, Vivithanaporn P, Siangcham T, Meemon K, Niamnont N, Sobhon P, et al. Hexane extract of seaweed Caulerpa lentillifera inhibits cell proliferation and induces apoptosis of human glioblastoma cells. STA. 2021;26(2):128-137.

Yuan YV, Walsh NA. Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol. 2006;44(7):1144-1150.

Namvar F, Mohamad R, Baharara J, Zafar-Balanejad S, Fargahi F, Rahman HS. Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). Biomed Res Int. 2013;2013: 604787.

Kong CS, Kim JA, Yoon NY, Kim SK. Induction of apoptosis by phloroglucinol derivative from Ecklonia Cava in MCF-7 human breast cancer cells. Food Chem Toxicol. 2009;47(7):1653-1658.

Fedorov SN, Ermakova SP, Zvyagintseva TN, Stonik VA. Anticancer and cancer preventive properties of marine polysaccharides: some results and prospects. Mar Drugs. 2013;11(12):4876-4901.

Maeda R, Ida T, Ihara H, Sakamoto T. Induction of apoptosis in MCF-7 cells by beta-1,3-xylooligosaccharides prepared from Caulerpa lentillifera. Biosci Biotechnol Biochem. 2012;76(5):1032-1034.

Ji H, Shao H, Zhang C, Hong P, Xiong H. Separation of the polysaccharides in Caulerpa racemosa and their chemical composition and antitumor activity. J Appl Polym Sci. 2008;110 (3):1435-1440.

Zhang M, Ma Y, Che X, Huang Z, Chen P, Xia G, et al. Comparative analysis of nutrient composition of Caulerpa lentillifera from different regions. J Ocean Univ China. 2020;19(2):439-445.

Namvar F, Tahir PM, Mohamad R, Mahdavi M, Abedi P, Najafi TF, et al. Biomedical properties of edible seaweed in cancer therapy and chemoprevention trials: a review. Nat Prod Commun. 2013;8(12): 1811-1820.