Comparison of In Vitro Anti-Inflammatory Activity of Extracts from Original Ya-Ha-Rak and Adapted Formula

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Atchara Kaewnoi
Supharat Duanyai
Omboon Vallisuta
Jantana Yahuafai

Abstract

This study aimed to compare the in vitro anti-inflammatory activity of the original Ya-Ha-Rak, and the adapted formula.  The original Ya-Ha-Rak formula is composed of the roots of Clerodendrum indicum (L.) Kuntze, Capparis micracantha DC., Ficus racemosa L., Tiliacora triandra Diels, and Harrisonia perforata Merr., whereas the adapted formula is similar to the original formula, except the root of H. perforata was replaced with the stem part.  The original Ya-Ha-Rak formula, and the adapted formula as well as each plant ingredient, were extracted with 95% ethanol and freeze-dried. The roots of H. perforata gave the highest extract yield of 4.26% w/w while that of H. perforata stem only gave 1.61% w/w. Dexamethasone was used as the positive control in the measuring of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. Our results demonstrated that the original Ya-Ha-Rak and the adapted formula significantly reduced nitric oxide production with IC50 values of 97.38±6.80 and 125.58±9.45 µg/mL respectively, in RAW 264.7 cells after treatment. At a 200 µg/mL dose of both formulas, the release of TNF-α and IL-1β was significantly reduced. The same results were obtained with dexamethasone (40 µM). Interestingly, the 5R extract demonstrated comparable results between PGE2 and dexamethasone. This study implied that the stem of H. perforata has the potential to be used in Ya-Ha-Rak provided that the appropriate ratio of the stem ingredient and its biological activities are further evaluated.

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References

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