Quantitative Analysis of Behavior in Rotenone-Induced Parkinsonism in Rats Using Force Plate Actimeter
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Abstract
Rotenone is a chemical that has been used to induce Parkinsonism-like behavioral symptoms such as tremor, bradykinesia, rigidity and postural instability in rats. In this study, we used rotenone to induce parkinsonism-like symptoms in rats. Eighteen Wistar rats were randomly divided into three groups: control group, 2.5 mg/kg, rotenone-treated group and 2.5 mg/kg rotenone+10 mg/kg Sinemetâ group. Rotenone was dissolved in soybean oil and injected subcutaneously every day excluding weekends. After 9 doses of soybean oil or rotenone, a series of oral doses of 3% (w/v) acacia or 10 mg/kg Sinemetâ was administered daily for 5 days, followed by a dose of acacia+soybean oil, acacia+rotenone, or Sinemetâ+rotenone. Animal behavior was assessed by using force plate actimeter. The results showed a significant decrease in motor ability of rats injected with rotenone compared to control group. Oral treatment of 10 mg/kg Sinemetâ improved the movement of rotenone-treated rats. Power spectra revealed that the parkinsonian features induced by rotenone possessed the frequency between 0.5-2.5 Hz and 4-12 Hz. This is the first model that applies the behavioral assessment using force plate actimeter in rotenone-induced parkinsonism in rats and thus may be used for evaluating new anti-parkinsonian drugs.
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