Induction of Apoptosis in Human B-lymphoma Cells by Citral

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Chatikorn Bunkrai
Tada Sueblinvong
Wacharee Limpanasithikul


This study intended to evaluate anti-tumor activity of citral on human B-lymphoma
cell line Ramos. Citral caused Ramos cell death in a concentration-dependent (37.5, 75 and
150 μM) as well as time dependent (3, 6 and 12 h) manner. By staining with annexin V-FITC
and propidium iodide (PI) and detecting with flow cytometer, citral induced cell death mostly
in apoptotic pattern. Other types of cell death were also detected only when the cells were
treated with high concentrations of citral for 6 and 12 h while apoptosis was still dominate.
Normal human peripheral blood mononuclear cells (PBMCs) were much more resistant than
Ramos cells to apoptotic induction by citral. These results demonstrated the beneficial effect
of citral which rich in Thai herbs and spices in oncology.

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2010 Annual Meeting Abstracts/Lectures