Potentiative Effects of Standardized Extract of Centella asiatica on Vinblastine-induced Cell Death

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Keywords:
Extract of Centella asiatica asiaticoside vinblastine LLC-PK1 LLC-MDR1

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Narumol Ruamsuk
Interdisciplinary Program in Pharmacology, Graduate School, Chulalongkorn University, Bangkok
Thitima Pengsuparp
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok
Suree Jianmongkol
Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok

Abstract

The purpose of this study was to investigate the influence of a standardized extract
from Centella asiatica (ECa-233) and asiaticoside (ATS) on vinblastine (VBL)-induced
cytotoxicity in porcine renal epithelial cell line (LLC-PK1) and its MDR1-gene-transfected
epithelial cell line (LLC-MDR1). The cells were co-treated with VBL (at various
concentrations) and either ECa-233 or ATS for 72 hr prior to determination of cell viability
with the MTT assay. Our results showed that the noncytotoxic ECa-233 and ATS could
enhance the cytotoxicity of VBL in both cell types. It was likely that both ECa-233 and ATS
were able to inhibit the function of P-glycoprotein, a known membrane efflux pump of VBL
and might be useful in cancer chemotherapy.

Article Details

Issue
Vol. 32 No. 1 (2010): Thai Journal of Pharmacology
Section
2010 Annual Meeting Abstracts/Lectures

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