Enhancing Cell Proliferation and Protection Hydrogen Peroxide-Induced Cytotoxicity in PC12 Cells by Rice Extracts

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Pimolvan Tappayuthpijarn
Arunporn Itharat
Suvaporn Saelim
Sakultala Uttama
Vanussaya Su-angkavatin


Rice is well-known as source of vitamin E, beta-glucan and gamma-oryzanol which
may be useful for the treatment of Alzheimer’s disease. Three varieties of rice, white rice:
white-hom-ma-li (MLW) and glong-hom-ma-li(MLG); red rice, Sung-yod (SY) were
extracted by various methods to obtain 14 fractions. Each fraction was studied for
cytotoxicity and protection neuronal cell from hydrogen peroxide-induced toxicity. By MTT
reduction assay, all fractions at dose of 100 μg/ml had no cytotoxic effect on PC12 cell
culture. Moreover, proliferative effect were detected only at dose of 50 μg/ml in lyophilized
rinse-water from SY (SYGW), MLG (MLGW), MLW(MLWW), ethanolic extract from
ML bran (MLBMEt ) as 119.33 , 119.69, 120.14, 112.41 respectively by 4 independence
experiments. At dose of 50 and 100 μg/ml, each fraction of MLWW, MLGW, ethanolic
extract of ML bran , MLG bran and SY bran (MLBMEt, MLGMEt SYBMEt), supercritical
extract from ML bran (MLBSUP), and cold-press of SY bran (SYBEX) could protect PC12
cells from oxidative stress induced by hydrogen peroxide with 83-92% effectiveness against
that of vitamin E. These results concluded that various fractions from rice: rinsed water,
ethanolic extract, supercritical extract and cold-press extract had protective effect on neuronal
cell PC12 from oxidative stress induce by hydrogen peroxide. This neuroprotective effect
may be due to the combination of more than one active principle in rice that could be
potential candidates for use as nutraceutical for neurodegenerative diseases.


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2010 Annual Meeting Abstracts/Lectures