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The objective of this research was investigating anti-inflammatory and antimicrobial
activities of Thai medicinal plants used to treat hemorrhoid. Inhibitory activity against LPS
induced NO production in RAW 264.7 cell lines was used as assay for anti-inflammation.
Disc diffusion method and MIC values were evaluated for antimicrobial against gram
positive (Staphylloccus aureus and Bacillus subtilis), gram negative (Escherichia coli) and
fungi or yeast (Candida albican). The extraction method, similar to folk doctors, used
maceration in ethanol and boiling in water. Five plants: Anacyclus pyrethrum (L.) DC.,
Angelica sylvestris Linn., Artemisia vulgaris Linn., Terminalia chebula Retz gall and
Picrorrhiza kurroa Royle. Ex Benth. in hemorrhoid preparation were selected to investigate.
The results were found that Artemisia vulgaris exhibited the most potent anti-inflammatory
activity with an IC50 value of 28.09 μg/ml and Terminalia chebula Retz gall extract showed
the highest antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and
Candida albicans with concentration of 0.28 mg/ml, 5 mg/ml and 1 mg/ml respectively.
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