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The study aimed to investigate acute toxicity and anti-cholangiocarcninoma activity
of the crude ethanolic extract of ginger (Zingiber officinale Roscoe). The extract was
resuspended in distilled water before given to hamsters (5 males and 5 females in each group)
via intragastric gavage. Ethanolic extract of ginger was shown absence of toxicity at the
maximum dose of 5,000 mg/kg body weight. The investigation of anti-cholangiocarcinoma
activity was carried out in Opisthorchis viverrini-dimethylnitrosamine (DMN) induced-cholangiocarcinoma (CCA) hamster model. The crude extract (at the dose of 1,000, 3,000
and 5,000 mg/kg body weight daily or every alternate day for 30 days) was fed to animals at
12 weeks after induction, with confirmation of cholangiocarcinoma by histopathological
examination at autopsy. Body weight, food and water consumption were recorded daily. The
pathogenesis change was examined by hematoxylin-eosin stained at autopsy. Study is
underway to conclude on the anticancer activity of the extract. The anti-oxidant activity was
also evaluated using the free radical, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. The
ethanolic extract of ginger exhibited moderate anti-oxidant activity with mean (SD) IC50
(concentration which produced 50% inhibition of oxidative activity) of 26.68 ± 0.16 μg/ml.
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