Inhibitory Effect of Harrisonia perforata Root Extract on Macrophage Activation

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Pattama Somsill
Chandhanee Itthipanichpong
Nijsiri Ruangrungsi
Wacharee Limpanasithikul

Abstract

Root of Harrisonia perforata Merr. (Simaroubaceae) is one of compositions in Thai
herbal remedy named Bencha-Loga-Wichien which has been used as an alternative medicine
for the treatment of pyresis. The direct effect of H.perforata on LPS-activated macrophage,
J774A.1 cells, were investigated in this study. The ethanol extract of H.perforata root
inhibited LPS-activated nitric oxide (NO) production in a concentration-dependent manner.
This inhibition was corresponded to the decrease in mRNA expression of inducible nitric
oxide systhase (iNOS) in the extract treated LPS-activated J774A.1 cells. The extract also
decrease the mRNA expression of the cyclooxygenase-2 (COX-2) which is the enzyme
required for large amount of prostaglandin synthesis in activated macrophage during
inflammatory process. These results indicated that the ethanol extract of H.perforata root has
direct inhibitory effect on macrophage activation. It may have not only antipyretic effect but
also anti-inflammatory activity.

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Section
2010 Annual Meeting Abstracts/Lectures